Krishan A, Pitman S W, Tattersall H N, Paika K D, Smith D C, Frei E
Cancer Res. 1976 Oct;36(10):3813-20.
A rapid propidium iodide staining method was used for analysis of single-cell suspensions of bone marrow and tumor biopsies by flow microfluorometry. With this technique, information on the proliferative status of target tissues can be obtained within 10 min of sample removal. DNA histograms and labeling index of sequential bone marrow biopsies from a patient with Stage IV diffuse lymphocytic leukemia and treated with 1-beta-D-arabinofuranosylcytosine infusion showed pronounced reduction in the percentage of cycling cells. In contrast, sequential tumor biopsies from a melanoma patient on methotrexate-citrovorum factor rescue therapy showed no changes. In sequential bone marrow biopsies of 3 patients on high-dose methotrexate-citrovorum factor rescue, initial accumulation of cells in G1-S (Day 1) was followed by a significant proliferative response (Days 4 to 7) and return to pretherapy values. In contrast, no recovery similar to that of the bone marrow was seen in tumor cells.
采用快速碘化丙啶染色法，通过流式微量荧光测定法分析骨髓和肿瘤活检组织的单细胞悬液。运用该技术，在取出样本后10分钟内即可获得有关靶组织增殖状态的信息。一名IV期弥漫性淋巴细胞白血病患者接受1-β-D-阿拉伯呋喃糖基胞嘧啶输注治疗，对其连续骨髓活检的DNA直方图和标记指数显示，循环细胞百分比显著降低。相比之下，一名接受甲氨蝶呤-亚叶酸解救治疗的黑色素瘤患者的连续肿瘤活检结果未显示出变化。在3名接受高剂量甲氨蝶呤-亚叶酸解救治疗患者的连续骨髓活检中，细胞在G1-S期初始蓄积（第1天）之后出现显著的增殖反应（第4至7天），随后恢复到治疗前水平。相比之下，肿瘤细胞未出现类似骨髓的恢复情况。
