通过在苯酚-乙酸-水体系中进行凝胶过滤对牛脊髓中致脑炎多肽进行分级分离。
Fractionation of encephalitogenic polypeptides from bovine spinal cord by gel filtration in phenol--acetic acid--water.
作者信息
Carnegie P R, Lumsden C E
出版信息
Immunology. 1967 Feb;12(2):133-45.
Abstract
Basic peptides capable of causing experimental allergic encephalomyelitis have been isolated from bovine spinal cord. From gel filtration experiments on Sephadex with phenol—acetic acid—water they appeared to have a molecular weight of about 3000. It is not known yet exactly how many of these basic polypeptides are encephalitogenic. Their predominant basic amino acid was lysine and there was a high content of glycine and serine. Reasons are given for the assumption that they are naturally occurring substances of central nervous tissue and that they are not artificial degradation products of extracted cord proteins. A possible physiological role for these compounds in myelin is discussed.
摘要
能够引发实验性变应性脑脊髓炎的碱性肽已从牛脊髓中分离出来。通过在葡聚糖凝胶上用苯酚—乙酸—水进行凝胶过滤实验，它们的分子量似乎约为3000。目前尚不清楚这些碱性多肽中有多少具有致脑炎性。它们主要的碱性氨基酸是赖氨酸，且甘氨酸和丝氨酸含量很高。文中给出了相关理由，认为它们是中枢神经组织的天然存在物质，而非提取的脊髓蛋白的人为降解产物。还讨论了这些化合物在髓鞘中可能的生理作用。
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