Vulchanov V H, Nikolov L S, Kehayov I R
Immunology. 1967 Mar;12(3):321-5.
Serum was obtained from fifty-one patients before operation for senile cataract. Individual extracts were prepared from the excised lenses and were used as autologous antigens in complement-fixation and precipitin tests upon the patients' sera. The complement-fixation test was positive with forty-three of the fifty-one sera and the precipitin tests with twenty-seven out of forty-five of the sera tested by this method. The incidence of both antibodies was higher in patients with cataract of relatively long duration, but complement-fixing antibody of titre 1:32 or more appears to develop earlier during the course of the disease than positive precipitin tests. The incidence of complement-fixation antibody was also relatively high in patients undergoing operation for a second cataract. No obvious correlation was observed between the two antibodies in individual sera but the most strongly positive precipitin reactions tended to be associated with negative or low titre complement-fixation tests. Serum from forty individuals without eye disease were also tested, using as antigen a pooled extract of many lenses; the results were completely negative.
从51例老年性白内障患者术前采集血清。从摘除的晶状体中制备个体提取物，并将其用作自身抗原，对患者血清进行补体结合试验和沉淀素试验。51份血清中有43份补体结合试验呈阳性，用该方法检测的45份血清中有27份沉淀素试验呈阳性。病程相对较长的白内障患者中两种抗体的发生率较高，但效价为1:32或更高的补体结合抗体似乎在疾病过程中比阳性沉淀素试验出现得更早。第二次白内障手术患者中补体结合抗体的发生率也相对较高。个体血清中两种抗体之间未观察到明显相关性，但最强阳性的沉淀素反应往往与阴性或低效价补体结合试验相关。还使用多种晶状体的混合提取物作为抗原，对40名无眼部疾病个体的血清进行了检测，结果均为阴性。
