Sokal J E, Ezdinli E Z
J Clin Invest. 1967 May;46(5):778-85. doi: 10.1172/JCI105578.
The isolated perfused rat liver can serve as a bioassay system for glucagon, capable of detecting 10 mmug of this agent. Seven 15-ml plasma specimens obtained from different healthy volunteers after an overnight fast were assayed in this system; glucagon could not be detected in any of them, indicating concentrations significantly below 0.67 mmug per ml in all subjects. The effects of administering small doses of glucagon to patients were consistent with these results; imposition of increments to plasma glucagon concentration below 1 mmug per ml induced distinct and sustained increases in blood glucose. Observations of the biologic effects of glucagon, together with data on the rate of its inactivation by the liver, suggest that the basal concentration of this hormone in peripheral plasma probably does not exceed 0.1 mmug per ml.
离体灌注大鼠肝脏可作为胰高血糖素的生物测定系统，能够检测到10毫微克该物质。从7名健康志愿者禁食过夜后采集的15毫升血浆样本在该系统中进行了检测；在任何样本中均未检测到胰高血糖素，表明所有受试者的浓度均显著低于每毫升0.67毫微克。给患者注射小剂量胰高血糖素的效果与这些结果一致；将血浆胰高血糖素浓度提高到每毫升1毫微克以下会导致血糖明显且持续升高。对胰高血糖素生物学效应的观察以及关于其在肝脏中失活速率的数据表明，外周血浆中该激素的基础浓度可能不超过每毫升0.1毫微克。
