酸在体外从人十二指肠释放胃动素。

Acid releases motilin from human duodenum in vitro.

作者信息

Strunz U, Mitznegg P, Domschke W, Subramanian N, Domschke S, Wünsch E

出版信息

Acta Hepatogastroenterol (Stuttg). 1977 Dec;24(6):456-7.

Abstract

The effect of hydrochloric acid and sodium hydroxide on motilin release from human duodenal mucosa was studied in vitro. Tissue was incubated in 2 ml chambers continuously perfused with medium and was exposed repeatedly to the stimuli. Motilin released into the medium was determined by a specific radioimmunoassay. Whereas alkalinisation abolished basal motilin release, acidification resulted in a 14-fold increase of peptide secretion. The results indicate that motilin secretion can be stimulated in vitro and that the perfusion system described may be a useful tool in studying the mechanism of gastrointestinal hormone release.

摘要

在体外研究了盐酸和氢氧化钠对人十二指肠黏膜胃动素释放的影响。将组织置于2毫升的小室中，小室持续灌注培养基，并反复暴露于刺激物。通过特异性放射免疫测定法测定释放到培养基中的胃动素。碱化可消除基础胃动素释放，而酸化则导致肽分泌增加14倍。结果表明，胃动素分泌在体外可被刺激，所述的灌注系统可能是研究胃肠激素释放机制的有用工具。