Weinert H, Masui H, Radichevich I, Werner S C
J Clin Invest. 1967 Jul;46(7):1264-74. doi: 10.1172/JCI105619.
Materials indistinguishable from authentic mono- and diiodotyrosines were identified in extracts of normal human serum as well as in extracts of purified human serum albumin. These materials were not found in association with the other serum proteins. Identification of MIT and DIT was made by a technique using rechromatography to constant specific activity, as well as by the Barker wet ash distillation method, which established the compounds in question as being iodinated ones. By two different extraction and chromatographic methods we estimated the amounts of both MIT and DIT present in normal human serum or albumin; the estimates were in good agreement. These compounds together constituted between 19% and 25% of the extractable serum iodine.
在正常人血清提取物以及纯化的人血清白蛋白提取物中，鉴定出了与正宗单碘酪氨酸和二碘酪氨酸无法区分的物质。这些物质未在其他血清蛋白中发现。通过使用再色谱法使比活性恒定的技术以及巴克湿灰化蒸馏法对MIT和DIT进行鉴定，该方法确定所讨论的化合物为碘化化合物。通过两种不同的提取和色谱方法，我们估计了正常人血清或白蛋白中MIT和DIT的含量；估计结果吻合良好。这些化合物共同构成了可提取血清碘的19%至25%。
