Johnson M J
J Bacteriol. 1967 Jul;94(1):101-8. doi: 10.1128/jb.94.1.101-108.1967.
Sterilizable membrane probes were used to study the relation between oxygen concentration and respiration rate in Candida utilis growing on acetate. When the organism was grown in a continuous fermentor at various dissolved oxygen concentrations (0.23 x 10(-6) to 32 x 10(-6)m), with time allowed for full adaptation to each oxygen concentration, the relationship between oxygen concentration and growth rate simulated Michaelis-Menten behavior, giving an apparent K(m) for oxygen of 1.3 x 10(-6)m. When respiration rate was measured at various oxygen concentrations without allowing time for adaptation, it was found that the respiration rate was directly proportional to O(2) concentration at low O(2) concentrations, and independent of O(2) concentration at high O(2) concentrations. Transition from one type of behavior to the other was fairly abrupt. The respiration rate in the presence of excess oxygen depended on the O(2) concentration at which the cells were grown, but the rate at low O(2) concentrations did not. There was evidence that, at low oxygen concentrations, oxygen diffusion through the cell substance limits respiration rate, at least in part.
可消毒的膜探针被用于研究产朊假丝酵母在乙酸盐上生长时氧气浓度与呼吸速率之间的关系。当该生物体在连续发酵罐中于不同的溶解氧浓度（0.23×10⁻⁶至32×10⁻⁶m）下生长，并给予足够时间使其完全适应每种氧气浓度时，氧气浓度与生长速率之间的关系模拟了米氏行为，得出氧气的表观K(m)为1.3×10⁻⁶m。当在不给予适应时间的情况下于不同氧气浓度下测量呼吸速率时，发现呼吸速率在低氧气浓度下与O₂浓度成正比，而在高氧气浓度下与O₂浓度无关。从一种行为类型向另一种行为类型的转变相当突然。在过量氧气存在下的呼吸速率取决于细胞生长时的O₂浓度，但在低O₂浓度下的速率则不然。有证据表明，在低氧气浓度下，氧气通过细胞物质的扩散至少在一定程度上限制了呼吸速率。
