Cell wall changes during the budding process of Paracoccidioides brasiliensis and Blastomyces dermatitidis.

The difference between the budding process of Paracoccidioides brasiliensis and Blastomyces dermatitidis is reported herein. A characteristic feature in P. brasiliensis is that the optical density of the cell wall increases at the site where budding begins and at the neck of the dividing cell, whereas B. dermatitidis does not undergo this alteration. The neck which is formed between the mother and daughter cell at the site of division is much wider in B. dermatitidis than in P. brasiliensis. The bud scar in P. brasiliensis appears as a truncated cone, the top of which is covered only by the inner layer of the cell wall; in comparison, in B. dermatitidis the bud scar exhibits a flattened surface covered by the cell wall. Both fungi show an increase in the number of mitochondria and infoldings of the cytoplasmic membrane at the site of separation, which indicates that at this site there is an increase of metabolic activity.