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氚标记胸腺嘧啶核苷：自身辐射分解对其作为DNA合成示踪剂特异性的影响。

Tritiated thymidine: effect of decomposition by self-radiolysis on specificity as a tracer for DNA synthesis.

作者信息

Wand M, Zeuthen E, Evans E A

出版信息

Science. 1967 Jul 28;157(3787):436-8. doi: 10.1126/science.157.3787.436.

DOI:10.1126/science.157.3787.436
PMID:6028027
Abstract

Tritiated thymidine undergoes decomposition by self-radiolysis. As shown by beta liquid-scintillation measurement and by autoradiography, the resultant breakdown products did not label DNA, but entered macromolecules, other than nucleic acid, contained in the cytoplasm. Caution should be exercised in using aged tritiated thymidine solutions.

摘要

氚标记胸腺嘧啶核苷会通过自身辐射分解。如通过β液体闪烁测量和放射自显影所示，产生的分解产物并未标记DNA，而是进入了细胞质中所含的除核酸之外的大分子中。在使用陈旧的氚标记胸腺嘧啶核苷溶液时应谨慎。

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Tritiated thymidine: effect of decomposition by self-radiolysis on specificity as a tracer for DNA synthesis.氚标记胸腺嘧啶核苷：自身辐射分解对其作为DNA合成示踪剂特异性的影响。
Science. 1967 Jul 28;157(3787):436-8. doi: 10.1126/science.157.3787.436.
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Mitochondrial incorporation of tritiated thymidine in Tetrahymena pyriformis.梨形四膜虫中线粒体对氚标记胸腺嘧啶核苷的摄取
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A periodicity of tritiated-thymidine incorporation into cytoplasmic deoxyribonucleic acid during the cell cycle of Tetrahymena pyriformis.在梨形四膜虫细胞周期中，氚标记胸腺嘧啶核苷掺入细胞质脱氧核糖核酸的周期性。
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DNA synthesis in relation to cell division in Tetrahymena pyriformis.梨形四膜虫中与细胞分裂相关的DNA合成
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[Synthesis of miochondrial DNA in Tetrahymena pyriformis. Electron microscopic radioautographic study].[梨形四膜虫线粒体DNA的合成。电子显微镜放射自显影研究]
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Synchronous DNA synthesis following heat-synchronized cell division in Tetrahymena.嗜热四膜虫热同步细胞分裂后的同步DNA合成。
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Zinc-deficient embryos: reduced thymidine incorporation.
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Genetic effects of tritiated thymidine and evidence for its incorporation into a cytoplasmic component of the adult testis of Drosophila melanogaster.氚标记胸腺嘧啶核苷的遗传效应及其掺入黑腹果蝇成年睾丸细胞质成分的证据。
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Effect of lithium and thiocyanate on the nucleic acid-synthesis of tetrahymena.锂和硫氰酸盐对四膜虫核酸合成的影响。
Naturwissenschaften. 1970 May;57(5):250. doi: 10.1007/BF01010282.

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治疗剂量的氚标记8-甲氧基补骨脂素经口给药后大鼠器官中放射性的定位。
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J Cell Biol. 1970 Sep;46(3):533-43. doi: 10.1083/jcb.46.3.533.
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Incorporation of labelled degradation products of radioactive thymine into non DNA material.放射性胸腺嘧啶标记的降解产物掺入非DNA物质中。
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