噬斑形成细胞的放射自显影研究。I. 抗原刺激下噬斑形成细胞的增殖
Radioautographic studies of plaque-forming cells. I. Antigen-stimulated proliferation of plaque-forming cells.
作者信息
Koros A M, Mazur J M, Mowery M J
出版信息
J Exp Med. 1968 Aug 1;128(2):235-57. doi: 10.1084/jem.128.2.235.
Abstract
A method has been described for obtaining radioautographs of plaque-forming cells. The method permits radioautographic analyses of small numbers of plaque-forming cells amidst large populations of non-plaque-forming cells. Spleen cells that were pulse-labeled with tritiated thymidine could be categorized readily as labeled or not labeled. Using this method it was found that (a) at least 55% of plaque-forming cells which appear 3 days after a maximal stimulus of 4 x 10(8) sheep red cells are still capable of DNA synthesis, and must have arisen by cell proliferation; (b) the rate of proliferation of plaque-forming cells is proportional to the log of the dose of antigen; (c) the S period of plaque-forming cells is at least 2 hr, appears to be constant, and is not influenced by antigen dose. The results suggest that antigen stimulates proliferation of plaque-forming cells by hastening their transit through the G(1) phase of the generative cycle.
摘要
已描述了一种获得噬斑形成细胞放射自显影照片的方法。该方法允许在大量非噬斑形成细胞中对少量噬斑形成细胞进行放射自显影分析。用氚标记的胸腺嘧啶核苷脉冲标记的脾细胞可很容易地分类为标记或未标记。使用该方法发现：（a）在4×10⁸个绵羊红细胞最大刺激后3天出现的噬斑形成细胞中，至少55%仍能够进行DNA合成，且必定是通过细胞增殖产生的；（b）噬斑形成细胞的增殖速率与抗原剂量的对数成正比；（c）噬斑形成细胞的S期至少为2小时，似乎是恒定的，且不受抗原剂量的影响。结果表明，抗原通过加速噬斑形成细胞在生成周期的G₁期的过渡来刺激其增殖。
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