Roede M J
Acta Morphol Neerl Scand. 1977 Dec;15(4):269-74.
The preparation and the utility of oral mucosa and hair root sheaths for quantitative sex chromatin studies is discussed. In buccal smears requirements for reliable scoring are frequently not fulfilled. Counting is time-consuming and disturbing measurement errors cannot be avoided. On the other hand, hair root sheath slides can be quickly prepared, the method yields preparations of good quality, and sex chromatin is displayed with considerable clarity. This procedure thus provides a high degree of accuracy and simplicity and counting can be quickly done with a relatively small measurement error. The incidence of sex chromatin in hair nuclei is observed to be about 20 per cent higher than in oral mucosa of the same female. In hair root sheaths non-peripheral sex chromatin can easily be recognized; including these into the scores significantly increases the actual incidence of sex chromatin positive nuclei.
本文讨论了用于定量性染色质研究的口腔黏膜和毛根鞘的制备方法及其用途。在颊黏膜涂片检查中，常常无法满足可靠评分的要求。计数耗时且不可避免地会出现干扰性测量误差。另一方面，毛根鞘玻片制备迅速，该方法能得到质量良好的标本，性染色质显示得相当清晰。因此，该方法具有高度的准确性和简便性，计数可快速完成，测量误差相对较小。观察发现，毛发细胞核中性染色质的发生率比同一位女性口腔黏膜中的发生率约高20%。在毛根鞘中，非周边性染色质很容易识别；将这些包括在评分中会显著提高性染色质阳性细胞核的实际发生率。
