Nagamori H
Nihon Hoigaku Zasshi. 1989 Oct;43(5):358-63.
The method and its reliability for sex determination by examining X-chromatin in the nucleus of hair root cell and buccal mucosa cell are presented: 1) X-chromatin in the nucleus of hair cortex cell was stained by Feulgen reaction after bleaching melanin granules and the frequency of X-chromatin was calculated under a ordinary microscope. An another method, fluorescent Feulgen reaction with acriflavine using a fluorescence microscope, was attempted. The results obtained from both methods indicated significantly higher values in the female samples than those in the male ones. 2) The other hand, the frequency of Y-chromatin was determined after quinacrine staining using a fluorescence microscope, and was also distinguishable between the male and female samples. 3) Using a single specimen, sex determination from the frequency of X- or Y-chromatin was practicable through combined treatment of quinacrine staining and fluorescent Feulgen reaction. 4) These methods were available during 16 to 64 weeks at least regarding samples kept dry. It is concluded that sex determination from frequency of sex chromatin after treatment described above is reliable.
介绍了通过检测发根细胞和口腔黏膜细胞核中的X染色质来进行性别鉴定的方法及其可靠性：1）在漂白黑色素颗粒后，用福尔根反应对毛发皮质细胞核中的X染色质进行染色，并在普通显微镜下计算X染色质的频率。还尝试了另一种方法，即使用荧光显微镜进行吖啶黄荧光福尔根反应。两种方法得到的结果均表明，女性样本中的值显著高于男性样本。2）另一方面，使用荧光显微镜在喹吖因染色后确定Y染色质的频率，男性和女性样本之间也有差异。3）使用单个样本，通过喹吖因染色和荧光福尔根反应的联合处理，根据X或Y染色质的频率进行性别鉴定是可行的。4）至少在16至64周内，对于干燥保存的样本，这些方法是有效的。结论是，通过上述处理后根据性染色质频率进行性别鉴定是可靠的。
