Jellinghaus W, Okada K, Ragg C, Gerhard H, Schröder F H
Invest Urol. 1976 Jul;14(1):16-9.
Chromosomal studies were performed on primary cultures from human prostatic adenomas and carcinomas. All cells from both tumor types showed diploid chromosomal counts with a normal human karyotype. Chromosomal analysis was also performed on cells of the permanent epithelial line EB 33 derived from a human prostatic carcinoma in this laboratory. In contrast to the primary cultures, hypotriploid chromosome numbers were found during continuous culture for more than 1 year. The range of chromosome numbers increased with time in culture and was reduced to the original model number of 64 by only one animal passage ("nude" mice). Feulgen fluorometry on different passages of the permanent cell line EB 33 showed tetraploidy. This discrepancy to the hypotriploid chromosome number can be explained by different Feulgen hydrolytic properties of the different cell types.
对人前列腺腺瘤和癌的原代培养物进行了染色体研究。两种肿瘤类型的所有细胞均显示二倍体染色体数，核型为正常人类核型。本实验室还对源自人前列腺癌的永久性上皮细胞系EB 33的细胞进行了染色体分析。与原代培养物不同，在连续培养1年以上的过程中发现了亚三倍体染色体数。染色体数范围随培养时间增加，仅经一次动物传代（“裸”鼠）就降至原来的64条模式数。对永久性细胞系EB 33不同传代的福尔根荧光测定显示为四倍体。这种与亚三倍体染色体数的差异可以用不同细胞类型不同的福尔根水解特性来解释。
