Schroeder F H, Okada K, Jellinghaus W, Wullstein H K, Heinemeyer H M
Invest Urol. 1976 May;13(6):395-403.
Tissues from human prostatic carcinomas and adenomas as well as cells from permanent cell lines originating from such tumors were transplanted into the subcutaneous space of "nude" mice. A total of 208 such transplantations were carried out. For transplantation of primary tumor tissue several techniques were used. Transplantation of single pieces of about 50 mg of tissue from carcinomas and adenomas provided the best results. With this technique carcinoma tissue was recovered from five of 10 tumors transplanted into 46 "nude" mice. Carcinoma was found in seven of 25 "nude" mice that were castrated and treated with testosterone but only once in 21 transplantations into castrates without testosterone substitution. This may suggest that heterotransplants of human prostatic carcinoma tissue can be established more easily in testosteronized animals. In the case of adenoma transplants, the histological picture was strikingly similar to the original tumors. Secretory activity was found in some acini, squamous metaplasia in others. Heterotransplantation of cells from permanent cell lines resulted in fast growing carcinomas without similarity to the origianl tumors. Dependence on hormones for growth of EB 33 and MA 160 cells in "nude" mice was not shown.
将人类前列腺癌和腺瘤组织以及源自此类肿瘤的永久细胞系的细胞移植到“裸”鼠的皮下空间。共进行了208次此类移植。对于原发性肿瘤组织的移植，使用了几种技术。移植约50毫克癌组织和腺瘤组织的单块组织效果最佳。采用这种技术，从移植到46只“裸”鼠体内的10个肿瘤中的5个中回收了癌组织。在25只去势并用睾酮治疗的“裸”鼠中，有7只发现了癌，但在21次移植到未用睾酮替代的去势鼠中仅发现1次。这可能表明，人前列腺癌组织异种移植在雄激素化动物中更容易建立。在腺瘤移植的情况下，组织学图像与原发肿瘤惊人地相似。在一些腺泡中发现了分泌活性，在另一些腺泡中发现了鳞状化生。源自永久细胞系的细胞异种移植导致快速生长的癌，与原发肿瘤不相似。未显示“裸”鼠中EB 33和MA 160细胞生长对激素的依赖性。
