Wright D E, Hungate R E
Appl Microbiol. 1967 Jan;15(1):148-51. doi: 10.1128/am.15.1.148-151.1967.
Methods using dialysis or ultrafiltration are described for the collection of extracellular fluid in rumen contents for analysis of amino acids. Marked differences in the concentration of aspartic acid, glutamic acid, and alanine were found in samples of either diffusate or ultrafiltrate and in clarified acidified rumen liquor. Concentrations are given for aspartic acid, glutamic acid, alanine, glycine, gamma-aminobutyric acid, valine, delta-aminovaleric acid, and leucine.
描述了使用透析或超滤的方法来收集瘤胃内容物中的细胞外液以分析氨基酸。在扩散液或超滤液样本以及澄清的酸化瘤胃液中发现了天冬氨酸、谷氨酸和丙氨酸浓度的显著差异。给出了天冬氨酸、谷氨酸、丙氨酸、甘氨酸、γ-氨基丁酸、缬氨酸、δ-氨基戊酸和亮氨酸的浓度。