Stubblefield R D, Shotwell O L, Hesseltine C W, Smith M L, Hall H H
Appl Microbiol. 1967 Jan;15(1):186-90. doi: 10.1128/am.15.1.186-190.1967.
A method has been developed for the production of aflatoxin by growing Aspergillus flavus NRRL 3145 on solid substrate wheat. Optimal yields of 900 mug of aflatoxin G(1) and 900 mug of aflatoxin B(1) per g of substrate were obtained in 4 to 5 days at 28 C. A study of aflatoxin production on hulls and groats of oats and on whole oats by A. flavus strains NRRL 2999, NRRL 3000, and NRRL 3145 revealed that aflatoxin was produced on all three substrates, although production was very slight on hulls. Strain NRRL 3145 grown on solid substrate groats produced the largest amounts of aflatoxin: 580 mug of B(1) and 450 mug of G(1) per g of substrate. A densitometric method for reading thin-layer chromatographic plates is described; this is more objective and more accurate than the visual methods previously used for the determination of all four aflatoxins.
已开发出一种通过在固体基质小麦上培养黄曲霉NRRL 3145来生产黄曲霉毒素的方法。在28℃下4至5天内，每克基质可获得900微克黄曲霉毒素G(1)和900微克黄曲霉毒素B(1)的最佳产量。对黄曲霉菌株NRRL 2999、NRRL 3000和NRRL 3145在燕麦壳、燕麦碎粒和整粒燕麦上产生黄曲霉毒素的研究表明，所有三种基质上均产生了黄曲霉毒素，尽管在燕麦壳上的产量非常低。在固体基质燕麦碎粒上生长的菌株NRRL 3145产生的黄曲霉毒素量最大：每克基质产生580微克B(1)和450微克G(1)。描述了一种用于读取薄层色谱板的光密度测定方法；该方法比以前用于测定所有四种黄曲霉毒素的目视方法更客观、更准确。
