Maidhof R, Prantl F, Hornstein O P, Schell H
Arch Dermatol Res (1975). 1977 Dec 27;260(3):193-200. doi: 10.1007/BF00561413.
In 29 patients, the DNA synthesis rate of clinically healthy buccal mucosa epithelium in comparison to benign buccal leukoplakias has been studied by in vitro-autoradiography. The proliferative activity has been determined by means of the 3H-labelling indices of basal cells (LBC) and suprabasal cells (LSBC) including the total of labelled epithelium cells and the quotient of LBC:LSBC as well. The progenitor compartment of both leukoplakic and normal buccal epithelium comprises the stratum basale and the adjacent 2-3 layers of suprabasal cells. Around 70% of the DNA synthesis of normal mucosa was found in the suprabasal nuclei of the progenitor compartment. In the leukoplakic mucosa, some displacement of the labelled nuclei in the progenitor compartment resulting in a statistically significant change of the quotient of LBC:LSBC in favour of LBC was determined. Moreover, the leukoplakic specimens showed a moderate decrease of the mean proliferative activity of the total of labelled epithelial cells which may be due to a diminished exfoliation of cornified cells from the hyper(ortho)keratotic surface. It is supposed that in chronic oral leukoplakias some variation of the steady state between renewal and desquamative loss of epithelial cells is operating as far as no precancerous condition is present.
通过体外放射自显影技术，对29例患者临床健康的颊黏膜上皮与良性颊黏膜白斑的DNA合成速率进行了研究。增殖活性通过基底细胞（LBC）和基底上层细胞（LSBC）的3H标记指数来确定，包括标记上皮细胞总数以及LBC:LSBC的比值。白斑和正常颊黏膜上皮的祖细胞区包括基底层和相邻的2 - 3层基底上层细胞。正常黏膜约70%的DNA合成发生在祖细胞区的基底上层细胞核中。在白斑黏膜中，确定了祖细胞区内标记细胞核的一些移位，导致LBC:LSBC比值发生统计学上显著的变化，有利于LBC。此外，白斑标本显示标记上皮细胞总数的平均增殖活性适度降低，这可能是由于角化过度（正角化）表面角质化细胞的脱落减少所致。据推测，在慢性口腔白斑中，只要不存在癌前状态，上皮细胞更新与脱屑性丢失之间的稳态就会发生一些变化。
