Liu S C, Klein-Szanto A J
Department of Pathology, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, PA 19006, USA.
Oral Oncol. 2000 Mar;36(2):145-51. doi: 10.1016/s1368-8375(99)00076-7.
We have reviewed the recent literature on immunohistochemical markers of cell proliferation in normal oral epithelia and leukoplakias. Most findings, including our own, point to an increased proliferation in oral leukoplakias that correlates with the degree of dysplasia. Although the basal layer of normal oral epithelia showed a very low proliferative activity, oral leukoplakias, even those containing low-grade dysplasia, exhibited a very significant increase in proliferation. High-grade dysplasia could be clearly differentiated from both low-grade dysplasia and normal oral epithelia by the presence of proliferating cells in the superficial cell strata, i.e. above or superficial to the parabasal layer. These changes were detected with several markers including PCNA, Ki-67 (Mib-1), cyclin D1 and CENP-F as well as with procedures using pulse labeling with BrDU, IrDU and tritiated thymidine. Comparison of all methods showed more similarities than discrepancies. Nevertheless, because of its relative simplicity of use and universal acceptance in many other sites, Ki-67 (Mib-1) seems to be the most reliable immunohistochemical marker for future use in cancer prevention and therapeutic clinical trials.
我们回顾了近期有关正常口腔上皮和白斑中细胞增殖免疫组化标志物的文献。包括我们自己的研究在内，大多数研究结果都表明口腔白斑中的增殖增加，且与发育异常程度相关。尽管正常口腔上皮的基底层显示出非常低的增殖活性，但口腔白斑，即使是那些含有低度发育异常的白斑，其增殖也显著增加。高级别发育异常可通过表层细胞层（即副基底层上方或表层）中增殖细胞的存在与低级别发育异常和正常口腔上皮明显区分开来。使用包括PCNA、Ki-67（Mib-1）、细胞周期蛋白D1和CENP-F在内的多种标志物以及使用溴脱氧尿苷（BrDU）、碘脱氧尿苷（IrDU）和氚标记胸腺嘧啶核苷进行脉冲标记的方法检测到了这些变化。所有方法的比较显示相似之处多于差异。然而，由于其使用相对简单且在许多其他部位被广泛接受，Ki-67（Mib-1）似乎是未来癌症预防和治疗临床试验中最可靠的免疫组化标志物。
