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氧分压或光照强度变化诱导的沼泽红假单胞菌胞内膜分化

Differentiation of the intracytoplasmic membrane of Rhodopseudomonas palustris induced by variations of oxygen partial pressure or light intensity.

作者信息

Firsow N N, Drews G

出版信息

Arch Microbiol. 1977 Dec 15;115(3):299-306. doi: 10.1007/BF00446456.

DOI:10.1007/BF00446456
PMID:603338
Abstract

The photosynthetic apparatus of Rhodopseudomonas palustris contains, in addition to reaction center bacteriochlorophyll (Bchl) two spectral forms of light harvesting (LH) Bchl, i.e. LH Bchl I, characterized by an infrared absorption maximum at 880 nm (890 nm at 77 degrees K) and LH Bchl II absorbing at 805 and 855 nm (805 and 870 nm at 77 degrees K). LH Bchl I seems to be associated with a single protein species of an apparent mol. wt. of 13 000 whereas LH Bchl II is apparently associated with two proteins of mol. wts. of 9000 and 11 000. Cells in anaerobic cultures adapt to changes of light intensity 1. by variation of the size of the photosynthetic unit, i.e. the molar ratio of LH Bchl II to reaction center Bchl, 2. by variation of the number of photosynthetic units per unit of membrane area, 3. by regulation of the size of the intracytoplasmic membrane system. During adaptation of changes of oxygen partial pressure cells are able to synthesize reaction center Bchl, LH Bchl and intracytoplasmic membranes at different rates. The synthesis of reaction center Bchl and LH Bchl I are, however, coordinated with each other, while the synthesis of LH Bchl II and reaction center Bchl proceed independently.

摘要

沼泽红假单胞菌的光合装置除了含有反应中心细菌叶绿素(Bchl)外,还含有两种光谱形式的捕光(LH)Bchl,即LH Bchl I,其特征在于在880nm处有一个红外吸收最大值(在77K时为890nm),以及在805和855nm处吸收的LH Bchl II(在77K时为805和870nm)。LH Bchl I似乎与一种表观分子量为13000的单一蛋白质相关,而LH Bchl II显然与两种分子量分别为9000和11000的蛋白质相关。厌氧培养的细胞通过以下方式适应光照强度的变化:1. 通过光合单位大小的变化,即LH Bchl II与反应中心Bchl的摩尔比;2. 通过单位膜面积内光合单位数量的变化;3. 通过调节胞内膜系统的大小。在适应氧分压变化的过程中,细胞能够以不同的速率合成反应中心Bchl、LH Bchl和胞内膜。然而,反应中心Bchl和LH Bchl I的合成相互协调,而LH Bchl II和反应中心Bchl的合成则独立进行。

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