从木醋杆菌中分离α-葡聚糖和脂多糖组分。
Isolation of alpha-glucan and lipopolysaccharide fractions from Acetobacter xylinum.
作者信息
Dekker R F, Rietschel E T, Sandermann H
出版信息
Arch Microbiol. 1977 Dec 15;115(3):353-7. doi: 10.1007/BF00446463.
A cellular phenol-water extract of Acetobacter xylinum NRC 17007 was fractionated on Sepharose 4 B. The fraction eluting with the void volume consisted to about 95% of glycogen-like material. The lipopolysaccharide fraction was of lower molecular weight and had the following composition (%, w/w): Mannose, 42; glucose, 7; galactose, 3.8; heptose, 2; 2-keto-3-deoxy-octonate, 1.2; glucosamine, 3.3; phosphate, 4.5; total fatty acids, 3.9. Among the fatty acids, 3-hydroxy-tetradecanoic acid was present, and 2-hydroxy-hexadecanoic acid predominated.
木醋杆菌NRC 17007的细胞酚水提取物在琼脂糖4B上进行分级分离。以空体积洗脱的级分约95%由类糖原物质组成。脂多糖级分分子量较低，其组成如下（重量百分比）：甘露糖42%；葡萄糖7%；半乳糖3.8%；庚糖2%；2-酮-3-脱氧辛酸1.2%；葡萄糖胺3.3%；磷酸盐4.5%；总脂肪酸3.9%。在脂肪酸中，存在3-羟基十四烷酸，且2-羟基十六烷酸占主导。
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