Rosenbaum J L, Child F M
J Cell Biol. 1967 Jul;34(1):345-64. doi: 10.1083/jcb.34.1.345.
The flagella of populations of three protozoan species (Ochromonas, Euglena, and Astasia) were amputated and allowed to regenerate. The kinetics of regeneration in all species were characterized by a lag phase during which there was no apparent flagellar elongation; this phase was followed by elongation at a rate which constantly decelerated as the original length was regained. Inhibition by cycloheximide applied at the time of flagellar amputation showed that flagellar regeneration was dependent upon de novo protein synthesis. This was supported by evidence showing that a greater amount of leucine was incorporated into the proteins of regenerating than nonregenerating flagella. The degree of inhibition of flagellar elongation observed with cycloheximide depended on how soon after flagellar amputation it was applied: when applied to cells immediately following amputation, elongation was almost completely inhibited, but its application at various times thereafter permitted considerable elongation to occur prior to complete inhibition of flagellar elongation. Hence, a sufficient number of precursors were synthesized and accumulated prior to addition of cycloheximide so that their assembly (elongation) could occur for a time under conditions in which protein synthesis had been inhibited. Evidence that the site of this assembly may be at the tip of the elongating flagellum was obtained from radioautographic studies in which the flagella of Ochromonas were permitted to regenerate part way in the absence of labeled leucine and to complete their regeneration in the presence of the isotope. Possible mechanisms which may be operating to control flagellar regeneration are discussed in light of these and other observations.
对三种原生动物（卵形鞭毛虫、眼虫和无色素眼虫）群体的鞭毛进行截肢，并使其再生。所有物种的再生动力学都具有一个延迟期，在此期间没有明显的鞭毛伸长；这个阶段之后是伸长，随着恢复到原来的长度，伸长速率不断减慢。在鞭毛截肢时应用环己酰亚胺进行抑制表明，鞭毛再生依赖于从头合成蛋白质。这得到了证据的支持，即与未再生的鞭毛相比，更多的亮氨酸被整合到再生鞭毛的蛋白质中。用环己酰亚胺观察到的鞭毛伸长抑制程度取决于在鞭毛截肢后多久应用它：在截肢后立即应用于细胞时，伸长几乎完全被抑制，但在此后不同时间应用时，在完全抑制鞭毛伸长之前允许相当程度的伸长发生。因此，在添加环己酰亚胺之前，已经合成并积累了足够数量的前体，以便在蛋白质合成被抑制的条件下，它们的组装（伸长）可以进行一段时间。从放射自显影研究中获得了证据，表明这种组装的位点可能在伸长的鞭毛尖端，在该研究中，卵形鞭毛虫的鞭毛在没有标记亮氨酸的情况下部分再生，并在同位素存在的情况下完成其再生。根据这些和其他观察结果，讨论了可能控制鞭毛再生的机制。
