Wood P D, Myers D, Lee Y L, Shioda R, Kinsell L W
J Lipid Res. 1967 Jul;8(4):406-8.
1,2-(3)H-Cholesterol was fed to a subject together with 4-(14)C-cholesterol at a known (3)H/(14)C ratio. The ratio was satisfactorily preserved in cholesterol recovered from plasma, red cells, and bile, and in bile acids of bile. Isotopic fractionation was seen during thin-layer chromatographic isolation of cholesterol. In work with 1,2-(3)H-cholesterol or its metabolites care should be taken to recover chromatographic bands in their entirety.
将1,2-(3)H-胆固醇与4-(14)C-胆固醇按已知的(3)H/(14)C比例喂给一名受试者。从血浆、红细胞和胆汁中回收的胆固醇以及胆汁中的胆汁酸中，该比例得到了令人满意的保持。在胆固醇的薄层色谱分离过程中观察到了同位素分馏现象。在使用1,2-(3)H-胆固醇或其代谢物时，应注意完整回收色谱带。
