氚标记胆固醇的不可靠性：[1,2-³H] -胆固醇和[24,25-³H] -胆固醇在人体中的研究
Unreliability of tritiated cholesterol: studies with [1,2-3H]-cholesterol and [24,25-3H]cholesterol in humans.
作者信息
Davidson N O, Ahrens E H, Bradlow H L, McNamara D J, Parker T S, Samuel P
出版信息
Proc Natl Acad Sci U S A. 1980 Apr;77(4):2255-9. doi: 10.1073/pnas.77.4.2255.
Abstract
Over the past 18 months different lots of [1,2-3H]cholesterol and [24,25-3H]cholesterol were found to be radiochemically acceptable by conventional chemical and other in vitro tests, yet, when co-administered with [4-14C]cholesterol to human subjects, an abrupt fall in the 3H/14C specific activity ratio in plasma cholesterol was discovered in every case. We have concluded that all batches of [3H]cholesterol should be regarded as radiochemically unreliable unless they are shown to behave identically in all respects to [4-14C]cholesterol in an appropriate in vivo assay system.
摘要
在过去18个月里，通过常规化学和其他体外试验发现，不同批次的[1,2 - 3H]胆固醇和[24,25 - 3H]胆固醇在放射化学方面是可接受的。然而，当与[4 - 14C]胆固醇共同给予人体受试者时，在每种情况下都发现血浆胆固醇中3H/14C比活度比值突然下降。我们得出结论，所有批次的[3H]胆固醇都应被视为放射化学不可靠，除非它们在适当的体内测定系统中在所有方面都表现得与[4 - 14C]胆固醇相同。
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