乙醛酸对柠檬酸循环的调控。草酰苹果酸和γ-羟基-α-酮戊二酸的抑制机制。
Control of the citric acid cycle by glyoxylate. Mechanism of the inhibition by oxalomalate and gamma-hydroxy-alpha-oxoglutarate.
作者信息
Ruffo A, Testa E, Adinolfi A, Pelizza G, Moratti R
出版信息
Biochem J. 1967 Apr;103(1):19-23. doi: 10.1042/bj1030019.
Abstract
- Hydroxyoxoglutarate was obtained by three methods: decarboxylation of oxalomalic acid, and synthesis from glyoxylate and pyruvate by using either Mg(2+) or an enzyme from rat liver as catalysts. 2. The inhibitory effects of oxalomalate and hydroxyoxoglutarate upon aconitate hydratase, isocitrate dehydrogenase (NADP) and oxoglutarate dehydrogenase were investigated. 3. Oxalomalate at low concentrations (1mm) inhibited almost completely both aconitate hydratase and isocitrate dehydrogenase. Hydroxyoxoglutarate also inhibited these enzymes, but at concentrations approximately tenfold that of oxalomalate. 4. Oxalomalate and hydroxyoxoglutarate, at the higher concentrations, inhibited oxoglutarate dehydrogenase to approximately the same extent. 5. It is suggested that the ability of glyoxylate to control reaction rates in the tricarboxylic acid cycle must in some degree be due to its condensation with oxaloacetate and pyruvate to form enzyme inhibitors.
摘要
- 羟基氧代戊二酸可通过三种方法获得：草酰苹果酸脱羧，以及以镁离子（Mg²⁺）或大鼠肝脏中的一种酶作为催化剂，由乙醛酸和丙酮酸合成。2. 研究了草酰苹果酸和羟基氧代戊二酸对乌头酸水合酶、异柠檬酸脱氢酶（NADP）和氧代戊二酸脱氢酶的抑制作用。3. 低浓度（1mM）的草酰苹果酸几乎完全抑制乌头酸水合酶和异柠檬酸脱氢酶。羟基氧代戊二酸也抑制这些酶，但所需浓度约为草酰苹果酸的十倍。4. 高浓度时，草酰苹果酸和羟基氧代戊二酸对氧代戊二酸脱氢酶的抑制程度大致相同。5. 有人提出，乙醛酸控制三羧酸循环中反应速率的能力在某种程度上必定归因于它与草酰乙酸和丙酮酸缩合形成酶抑制剂。
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