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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Diliberto E J, Davis R, Boelle G B

Gen Pharmacol. 1976 Sep;7(4):239-48. doi: 10.1016/0306-3623(76)90082-3.

The localization of acetylcholinesterase (AChE) was studied by light and electron microscopy (LM and EM) at the neuromuscular junctions (NMJs) of the abdominal superficial flexor muscle (SFM) and the claw adductor muscle (CAM) of the crayfish in view of reported pharmacological evidence of cholinergic transmission at the former but not at the latter site. 2. Survey of LM with the copper-thiocholine method disclosed staining for AChE, but not for butyrocholinesterase (BuChE), at numerous NMJs of the SFM; neither enzyme was detected at any NMJs of the CAM. 3. With the bis-(thioacetoxy) aurate (I) method, EM examination showed AChE-staining at NMJs of the SFM to be confined to the postjunctional membrane. 4. The published and present findings suggest a unique situation of more than one excitatory transmitter for the skeletal neuromuscular system in the crustacean studied.

鉴于有报道称在小龙虾腹部浅屈肌（SFM）的神经肌肉接头（NMJ）处存在胆碱能传递的药理学证据，而在爪内收肌（CAM）处则不存在，故采用光镜和电镜（LM和EM）研究了乙酰胆碱酯酶（AChE）在这两种肌肉神经肌肉接头处的定位。2. 用硫代胆碱铜法进行光镜观察发现，在SFM的许多神经肌肉接头处有AChE染色，但无丁酰胆碱酯酶（BuChE）染色；在CAM的任何神经肌肉接头处均未检测到这两种酶。3. 用双（硫代乙酰氧基）金（I）法进行电镜检查显示，SFM神经肌肉接头处的AChE染色局限于突触后膜。4. 已发表的研究结果和目前的研究结果表明，在所研究的甲壳类动物中，其骨骼神经肌肉系统存在一种独特的情况，即存在不止一种兴奋性递质。

Diliberto E J, Davis R, Boelle G B

Gen Pharmacol. 1976 Sep;7(4):239-48. doi: 10.1016/0306-3623(76)90082-3.

The localization of acetylcholinesterase (AChE) was studied by light and electron microscopy (LM and EM) at the neuromuscular junctions (NMJs) of the abdominal superficial flexor muscle (SFM) and the claw adductor muscle (CAM) of the crayfish in view of reported pharmacological evidence of cholinergic transmission at the former but not at the latter site. 2. Survey of LM with the copper-thiocholine method disclosed staining for AChE, but not for butyrocholinesterase (BuChE), at numerous NMJs of the SFM; neither enzyme was detected at any NMJs of the CAM. 3. With the bis-(thioacetoxy) aurate (I) method, EM examination showed AChE-staining at NMJs of the SFM to be confined to the postjunctional membrane. 4. The published and present findings suggest a unique situation of more than one excitatory transmitter for the skeletal neuromuscular system in the crustacean studied.

鉴于有报道称在小龙虾腹部浅屈肌（SFM）的神经肌肉接头（NMJ）处存在胆碱能传递的药理学证据，而在爪内收肌（CAM）处则不存在，故采用光镜和电镜（LM和EM）研究了乙酰胆碱酯酶（AChE）在这两种肌肉神经肌肉接头处的定位。2. 用硫代胆碱铜法进行光镜观察发现，在SFM的许多神经肌肉接头处有AChE染色，但无丁酰胆碱酯酶（BuChE）染色；在CAM的任何神经肌肉接头处均未检测到这两种酶。3. 用双（硫代乙酰氧基）金（I）法进行电镜检查显示，SFM神经肌肉接头处的AChE染色局限于突触后膜。4. 已发表的研究结果和目前的研究结果表明，在所研究的甲壳类动物中，其骨骼神经肌肉系统存在一种独特的情况，即存在不止一种兴奋性递质。