Ling G N, Ochsenfeld M M, Karreman G
J Gen Physiol. 1967 Jul;50(6):1807-20. doi: 10.1085/jgp.50.6.1807.
With the use of a newly introduced technique, the "influx profile analysis," we studied the diffusion of tritiated water in and out of frog ovarian eggs at 25 degrees C. The results show that the rate-limiting step in the exchange of labeled water is not permeation through the cell membrane but diffusion in the bulk of the intracellular water.
通过使用一种新引入的技术——“流入剖面分析”,我们研究了氚标记水在25摄氏度下进出青蛙卵巢卵的扩散情况。结果表明,标记水交换中的限速步骤不是通过细胞膜的渗透,而是在细胞内大量水中的扩散。