豚鼠大脑皮层中己糖激酶的亚细胞分布及特性
The subcellular distribution and properties of hexokinases in the guinea-pig cerebral cortex.
作者信息
Bachelard H S
出版信息
Biochem J. 1967 Jul;104(1):286-92. doi: 10.1042/bj1040286.
Abstract
- Hexokinase activities were estimated in primary subcellular fractions from guinea-pig cerebral cortex and in sucrose-density-gradient subfractions of the mitochondrial and microsomal fractions. 2. Appreciable activities were observed in mitochondrial, microsomal and soluble fractions. The activity in the mitochondrial fraction was associated with the mitochondria rather than with myelin or nerve endings and that in the microsomal fraction was associated with membrane fragments. 3. Most of the mitochondrial activity was extracted in soluble form by osmotic ;shock'. The activity of the mitochondrial extract differed from the soluble activity in kinetic properties and in electrophoretic behaviour. 4. No evidence was obtained for the presence of a high-K(m) glucokinase in the brain. 5. The results are discussed in terms of relevance to considerations of glucose utilization by the brain.
摘要
- 对豚鼠大脑皮层初级亚细胞组分以及线粒体和微粒体组分的蔗糖密度梯度亚组分中的己糖激酶活性进行了评估。2. 在线粒体、微粒体和可溶性组分中观察到了明显的活性。线粒体组分中的活性与线粒体相关，而非与髓磷脂或神经末梢相关，微粒体组分中的活性与膜碎片相关。3. 大部分线粒体活性通过渗透“休克”以可溶形式提取出来。线粒体提取物的活性在动力学性质和电泳行为上与可溶性活性不同。4. 未获得大脑中存在高K（m）葡萄糖激酶的证据。5. 根据与大脑葡萄糖利用相关的考虑对结果进行了讨论。
相似文献
1
The subcellular distribution and properties of hexokinases in the guinea-pig cerebral cortex.豚鼠大脑皮层中己糖激酶的亚细胞分布及特性
Biochem J. 1967 Jul;104(1):286-92. doi: 10.1042/bj1040286.
2
Factors affecting the glucose 6-phosphate inhibition of hexokinase from cerebral cortex tissue of the guinea pig.影响豚鼠大脑皮层组织中己糖激酶的6-磷酸葡萄糖抑制作用的因素。
Biochem J. 1968 Jan;106(1):193-201. doi: 10.1042/bj1060193.
3
Cerebral-cortex hexokinase. Comparison of properties of solubilized mitochondrial and cytoplasmic activities.大脑皮质己糖激酶。可溶性线粒体活性与细胞质活性的性质比较。
Biochem J. 1970 Jun;118(1):25-34. doi: 10.1042/bj1180025.
4
Subcellular localization of hexokinase in the rat cortex.己糖激酶在大鼠皮层中的亚细胞定位。
Neurosci Behav Physiol. 1973 Jul-Sep;6(3):218-27. doi: 10.1007/BF01190345.
5
Subcellular distribution and properties of carbonyl reductase in guinea pig lung.豚鼠肺中羰基还原酶的亚细胞分布及特性
Arch Biochem Biophys. 1988 Aug 1;264(2):492-501. doi: 10.1016/0003-9861(88)90314-1.
6
Differences in catalytic properties between cerebral cytoplasmic and mitochondrial hexokinases.脑细胞质和线粒体己糖激酶催化特性的差异。
Biochem J. 1977 Mar 1;161(3):593-8. doi: 10.1042/bj1610593.
7
8
The localization of adenosine triphosphatases in morphologically characterized subcellular fractions of guinea-pig brain.豚鼠脑形态学特征亚细胞组分中三磷酸腺苷酶的定位
Biochem J. 1965 Aug;96(2):404-12. doi: 10.1042/bj0960404.
9
Adenosine triphosphate:creatine phosphotransferase of rat cerebral cortex: evidence for a bimodal subcellular localization.大鼠大脑皮质三磷酸腺苷：肌酸磷酸转移酶：双峰亚细胞定位的证据
J Neurochem. 1968 Feb;15(2):115-21. doi: 10.1111/j.1471-4159.1968.tb06182.x.
10
Cerebral-cortex hexokinase. Elucidation of reaction mechanisms by substrate and dead-end inhibitor kinetic analysis.大脑皮质己糖激酶。通过底物和终产物抑制剂动力学分析阐明反应机制。
Biochem J. 1971 Aug;123(5):707-15. doi: 10.1042/bj1230707.
引用本文的文献
1
Fueling and imaging brain activation.为大脑激活提供燃料和成像。
ASN Neuro. 2012 Jul 20;4(5):e00093. doi: 10.1042/AN20120021.
2
Adenine nucleotides and magnesium ions in relation to control of mammalian cerebral-cortex hexokinase.腺嘌呤核苷酸和镁离子与哺乳动物大脑皮层己糖激酶的调控关系
Biochem J. 1969 May;112(5):579-86. doi: 10.1042/bj1120579.
3
Uptake of monosaccharides by guinea-pig cerebral-cortex slices.豚鼠大脑皮层切片对单糖的摄取
Biochem J. 1969 Apr;112(3):367-71. doi: 10.1042/bj1120367.
4
Solubilization of mitochondrial hexokinase from ox cerebral cortex with Triton X-100.用曲拉通X-100增溶牛大脑皮质中的线粒体己糖激酶。
Biochem J. 1969 Feb;111(3):18P-19P. doi: 10.1042/bj1110018pb.
5
Glucose metabolism in the mucosa of the small intestine. A study of hexokinase activity.小肠黏膜中的葡萄糖代谢。己糖激酶活性研究。
Biochem J. 1968 Aug;109(1):35-42. doi: 10.1042/bj1090035.
6
Cerebral-cortex hexokinase. Comparison of properties of solubilized mitochondrial and cytoplasmic activities.大脑皮质己糖激酶。可溶性线粒体活性与细胞质活性的性质比较。
Biochem J. 1970 Jun;118(1):25-34. doi: 10.1042/bj1180025.
7
Subcellular localization of hexokinase in the rat cortex.己糖激酶在大鼠皮层中的亚细胞定位。
Neurosci Behav Physiol. 1973 Jul-Sep;6(3):218-27. doi: 10.1007/BF01190345.
8
Enzymes of glucose metabolism in cultured human gliomas: neoplasia is accompanied by altered hexokinase, phosphofructokinase, and glucose-6-phosphate dehydrogenase levels.培养的人脑胶质瘤中葡萄糖代谢的酶：肿瘤形成伴随着己糖激酶、磷酸果糖激酶和葡萄糖-6-磷酸脱氢酶水平的改变。
Metab Brain Dis. 1987 Mar;2(1):17-30. doi: 10.1007/BF00999506.
9
Development of mitochondrial energy metabolism in rat brain.大鼠脑线粒体能量代谢的发育
Biochem J. 1977 May 15;164(2):339-48. doi: 10.1042/bj1640339.
10
Differences in catalytic properties between cerebral cytoplasmic and mitochondrial hexokinases.脑细胞质和线粒体己糖激酶催化特性的差异。
Biochem J. 1977 Mar 1;161(3):593-8. doi: 10.1042/bj1610593.
本文引用的文献
1
The inhibition of hexokinase by disulphides.二硫化物对己糖激酶的抑制作用。
Biochem J. 1963 Jun;87(3):526-32. doi: 10.1042/bj0870526.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
CONTINUOUS RECORDINGS OF CHANGES IN MEMBRANE POTENTIAL IN MAMMALIAN CEREBRAL TISSUES IN VITRO; RECOVERY AFTER DEPOLARIZATION BY ADDED SUBSTANCES.哺乳动物脑组织体外膜电位变化的连续记录；添加物质去极化后的恢复
J Physiol. 1965 Jan;176(2):261-83. doi: 10.1113/jphysiol.1965.sp007549.
4
EFFECT OF ISCHEMIA ON KNOWN SUBSTRATES AND COFACTORS OF THE GLYCOLYTIC PATHWAY IN BRAIN.缺血对脑糖酵解途径已知底物和辅助因子的影响。
J Biol Chem. 1964 Jan;239:18-30.
5
INSULIN-DEPENDENT SYNTHESIS OF LIVER GLUCOKINASE IN THE RAT.大鼠肝脏葡萄糖激酶的胰岛素依赖性合成
J Biol Chem. 1963 Nov;238:3535-8.
6
ON THE PRESENCE OF TWO SOLUBLE GLUCOSE-PHOSPHORYLATING ENZYMES IN ADULT LIVER AND THE DEVELOPMENT OF ONE OF THESE AFTER BIRTH.论成年肝脏中两种可溶性葡萄糖磷酸化酶的存在以及其中一种在出生后的发育情况。
Biochim Biophys Acta. 1963 Oct 1;77:209-26. doi: 10.1016/0006-3002(63)90494-3.
7
EFFECTS OF DIET AND INSULIN ON GLUCOSE-ADENOSINE TRIPHOSPHATE PHOSPHOTRANSFERASES OF RAT LIVER.饮食与胰岛素对大鼠肝脏葡萄糖 - 三磷酸腺苷磷酸转移酶的影响。
J Biol Chem. 1963 Dec;238:3840-5.
8
ROLE OF NON-COVALENT BONDS FOR THE HOLDING, ACTIVATION AND RELEASE OF MITOCHONDRIAL ENZYMES.非共价键在维持、激活和释放线粒体酶中的作用。
Nature. 1963 Oct 19;200:240-2. doi: 10.1038/200240a0.
9
The sodium-stimulated adenosine-triphosphatase activity and other properties of cerebral microsomal fractions and subfractions.脑微粒体组分及亚组分的钠刺激三磷酸腺苷酶活性和其他特性。
Biochem J. 1962 Sep;84(3):626-37. doi: 10.1042/bj0840626.
10
Kinetic studies of the brain hexokinase reaction.大脑己糖激酶反应的动力学研究。
J Biol Chem. 1962 May;237:1661-7.
Zotero 插件