一种改进的DNA碱性梯度分析方法及其在致癌物对小鼠肝脏DNA影响研究中的应用。
An improved method for DNA alkaline gradient analysis and its application to the effect of carcinogens on mouse liver DNA.
作者信息
Szafarz D
出版信息
Biochimie. 1977;59(10):775-8. doi: 10.1016/s0300-9084(77)80206-x.
PMID:603788
Abstract
An alkaline sodium iodide density gradient technique is described, for use in sedimentation rate centrifugation studies of in vivo induction of single strand breaks in DNA. The combination of this type of gradient with a sensitive fluorometric DNA estimation makes it possible to analyze very small amounts of DNA without any need for labeling the nucleic acid with radioactive thymidine.
摘要
本文描述了一种碱性碘化钠密度梯度技术，用于体内DNA单链断裂诱导的沉降速率离心研究。这种类型的梯度与灵敏的荧光DNA定量方法相结合，使得无需用放射性胸苷标记核酸就能够分析极少量的DNA。
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