Larrue J, Desgranges C, Daret D, Bricaud H
Cardiovasc Res. 1977 Nov;11(6):519-28. doi: 10.1093/cvr/11.6.519.
Aortic explants obtained from normal adult rats and composed of all 3 tunics were cultured in a semi-synthetic gelosed medium supplemented by 10% serum. Explants cultured with normocholesterolaemic serum kept their in vivo characteristics for more than 12 days at electron microscope and histometabolic levels. However, explants showed considerable differences when cultured in a medium with hypercholesterolaemic serum. Cell proliferation in the tunica media and enhanced synthesis of macromolecular components of the extracellular matrix were observed. These laterations occurring inside the explants, which had kept all their tissular relationships, suggest that this culture system may be valuable for further studies on atherosclerotic processes.
从正常成年大鼠获取的由所有三层膜组成的主动脉外植体，在添加10%血清的半合成凝胶化培养基中培养。用正常胆固醇血症血清培养的外植体在电子显微镜和组织代谢水平上保持其体内特征超过12天。然而，当在高胆固醇血症血清培养基中培养时，外植体显示出相当大的差异。观察到中膜细胞增殖以及细胞外基质大分子成分的合成增强。这些在外植体内发生的变化，外植体保持了其所有组织关系，表明该培养系统可能对动脉粥样硬化过程的进一步研究有价值。
