Larrue J, Daret D, Demond J, Bricaud H
Atherosclerosis. 1977 Sep;28(1):53-60. doi: 10.1016/0021-9150(77)90198-8.
Aortic tissues consisting of all three tunics were removed from normal adult rabbits and cultured in a semisynthetic gelosed medium supplemented by 10% serum obtained either from normal or hypercholesterolemic rabbits. Fibrillar cross-striated aggregates appeared with a high frequency (50%) in the extracellular space of explants cultured from four to eight days in medium supplemented by serum from hypercholesterolemic rabbits, but did not appear in explants cultured in serum from control animals (3%). The electron-dense segment was ruthenium red positive and digested by testicular hyaluronidase. The electron-lucent segment, composed of ruthenium red negative thin filaments, was not modified after hyaluronidase treatment but was strongly digested after collagenase treatment. It is believed that this material was fibrous long spacing collagen synthetized under culture conditions, as shown after tritiated proline incorporation.
从正常成年兔身上取出包含所有三层膜的主动脉组织，在添加了10%血清的半合成凝胶化培养基中培养，血清取自正常或高胆固醇血症兔。在添加了高胆固醇血症兔血清的培养基中培养4至8天的外植体的细胞外空间中，纤维状横纹聚集物高频出现（50%），但在对照动物血清中培养的外植体中未出现（3%）。电子致密段钌红染色呈阳性，可被睾丸透明质酸酶消化。由钌红阴性细丝组成的电子透明段，在透明质酸酶处理后未发生改变，但在胶原酶处理后被强烈消化。据信，这种物质是在培养条件下合成的纤维状长间距胶原，如氚标记脯氨酸掺入后所示。
