Norris W E
Department of Biology, Southwest Texas State College, San Marcos, Texas 78666.
Plant Physiol. 1967 Apr;42(4):481-6. doi: 10.1104/pp.42.4.481.
Hydroxy-l-proline-induced inhibition of elongation of Avena coleoptile segments was measured in water and in indole-3-acetic acid. This inhibition was completely reversed by l-proline.Time-sequence experiments revealed that some time had to elapse, or some elongation had to occur, prior to the onset of hydroxyproline inhibition. The presence of sucrose supported the rate of auxin-induced elongation throughout a 24-hour period, and enhanced the effectiveness of hydroxyproline as an inhibitor of elongation.The application of adenosine triphosphate effectively counteracted the hydroxy-proline-induced inhibition of elongation; completely in water, and partially in indole-3-acetic acid. Optimal ATP concentrations were between 0.25 and 0.75 mm. Similarly, guanine-HCL, l-glutamic acid, and l-ornithine-HCL were capable of reversing the hydroxyproline inhibition. Other compounds that were tested but which proved to be less effective are tabulated.It is suggested that hydroxyproline may exert its inhibitory effect on the protein and/or RNA synthesis necessary for elongation of the oat coleoptile by interference with the metabolism of adenosine triphosphate or other high energy compounds.
在水和吲哚 - 3 - 乙酸中测定了羟脯氨酸对燕麦胚芽鞘切段伸长的抑制作用。这种抑制作用被脯氨酸完全逆转。时间序列实验表明，在羟脯氨酸抑制作用开始之前，必须经过一段时间或发生一定程度的伸长。蔗糖的存在在整个24小时期间维持了生长素诱导的伸长速率，并增强了羟脯氨酸作为伸长抑制剂的效力。三磷酸腺苷的应用有效地抵消了羟脯氨酸诱导的伸长抑制；在水中完全抵消，在吲哚 - 3 - 乙酸中部分抵消。最佳ATP浓度在0.25至0.75毫米之间。同样，盐酸鸟嘌呤、L - 谷氨酸和盐酸L - 鸟氨酸能够逆转羟脯氨酸的抑制作用。其他经过测试但效果较差的化合物列于表中。有人提出，羟脯氨酸可能通过干扰三磷酸腺苷或其他高能化合物的代谢，对燕麦胚芽鞘伸长所需的蛋白质和/或RNA合成发挥抑制作用。
