Barme M
Dev Biol Stand. 1977;39:213-7.
Counterimmunoelectrophoresis was used for the detection of ovalbumin in influenza vaccine preparations throughout a purification process applied to allantoic fluids of embryonated eggs. With an antiserum able to detect ovalbumin to a concentration of 0.31 microgram/ml, the progressive elimination of this contaminant was followed until the final preparation of purified concentrated vaccine. This easy procedure appeared to be specific and highly sensitive.
在对鸡胚尿囊液进行纯化制备流感疫苗的整个过程中,采用对流免疫电泳法检测各阶段疫苗制剂中的卵清蛋白。使用能检测浓度低至0.31微克/毫升卵清蛋白的抗血清,跟踪该污染物在纯化浓缩疫苗最终制剂前的逐步去除过程。这一简便方法显示出特异性强且灵敏度高的特点。