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[纯化流感病毒制剂中卵清蛋白不同测定方法的比较研究]

[Comparative study of different methods of determining ovalbumin in preparations of purified influenza virus].

作者信息

Bichurina M A, Goriunova V V, Chubarova N I, Zhebruk A B, Noskov F S

出版信息

Vopr Virusol. 1981 Mar-Apr(2):228-30.

PMID:6791386
Abstract

The degree of purity of inactivated influenza vaccines was assayed in the anaphylaxis test in guinea pigs as well as by the values of total protein and ovalbumin measured by IHA and ID tests. Quantitation of ovalbumin in vaccines by the IHA test with an antiovalbumin diagnostic preparation was found to be most informative, rapid and highly sensitive. This method may be recommended for quantitation of ovalbumin impurities in samples of purified influenza virus obtained by different methods and for different purposes.

摘要

通过豚鼠过敏试验以及免疫血凝试验(IHA)和免疫扩散试验(ID)测定的总蛋白和卵清蛋白值,对灭活流感疫苗的纯度进行了测定。发现用抗卵清蛋白诊断制剂通过IHA试验对疫苗中的卵清蛋白进行定量分析,信息量大、速度快且灵敏度高。该方法可推荐用于对通过不同方法获得的、用于不同目的的纯化流感病毒样品中的卵清蛋白杂质进行定量分析。

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