血红素羰基的碳-13核磁共振。细胞色素c及细胞色素c的羧甲基衍生物。

Carbon-13 nuclear magnetic resonance of heme carbonyls. Cytochrome c and carboxymethyl derivatives of cytochrome c.

作者信息

Morgan L O, Eakin R T, Vergamimi P J, Matwiyoff N A

出版信息

Biochemistry. 1976 May 18;15(10):2203-7. doi: 10.1021/bi00655a027.

Abstract

Carbonyl complexes of horse cytochrome c and various carboxymethylated derivatives have been examined using 13C NMR (carbon-13 nuclear magnetic resonance) spectroscopy. The multiplicity and chemical shift of the 13CO resonance were found to be functions of pH and the extent of alkylation. Correlations have been made among prominent features of the chemical shift titration curves and changes in the environment of the heme. A simple model compatible with the bulk of previous observations has been suggested to account for the several carbonyl resonance peaks and the complex behavior of the chemical shift with changes in pH.

摘要

已使用13C核磁共振光谱法对马细胞色素c及各种羧甲基化衍生物的羰基配合物进行了研究。发现13CO共振的多重性和化学位移是pH值和烷基化程度的函数。已对化学位移滴定曲线的显著特征与血红素环境变化之间进行了关联。已提出一个与先前大多数观察结果相符的简单模型，以解释几个羰基共振峰以及化学位移随pH值变化的复杂行为。