Horikawa M, Chisaka N, Yokoyama S, Onoé T
J Histochem Cytochem. 1976 Aug;24(8):926-32. doi: 10.1177/24.8.60440.
When the immunofluroscent study on the distribution and the incidence of albumin-producing hepatocytes in the rat liver was performed by the method of Sainte-Marie, the number of positive cells showed various values (10-60%). It was surmised that when the permeability of the fixative was delayed, albumin had flowed out from the cytoplasm of the unfixed hepatocytes. By the simple means of constant stirring of the fixative using a magnetic stirrer, we accomplished rapid fixation and achieved results in which positive cells attained 100%. On the other hand, the incidence of positive cells decreased markedly when rats were fed a protein-free diet.
当采用圣玛丽方法对大鼠肝脏中产生白蛋白的肝细胞的分布和发生率进行免疫荧光研究时，阳性细胞数量呈现出不同的值（10 - 60%）。据推测，当固定剂的渗透延迟时，白蛋白已从未固定肝细胞的细胞质中流出。通过使用磁力搅拌器对固定剂进行简单的持续搅拌，我们实现了快速固定，并获得了阳性细胞达到100%的结果。另一方面，当给大鼠喂食无蛋白饮食时，阳性细胞的发生率显著降低。
