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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Quinn P J, White I G

Biochem J. 1967 Aug;104(2):328-31. doi: 10.1042/bj1040328.

When dog semen is stored at 5 degrees for 24hr., K(+) is lost from the spermatozoa and Na(+) accumulates in the cells. 2. If at the end of the cold-storage period the semen is incubated at 37 degrees in the presence of added glucose there is a rapid uptake of K(+) and extrusion of Na(+) from the spermatozoa, the intracellular K(+) reaching a maximum within 30min. 3. When the semen is incubated at 20 degrees after cold storage there is an uptake of K(+) by the spermatozoa over 3hr. but no change in intracellular Na(+) concentration. 4. The extrusion of Na(+) and uptake of K(+) by dog spermatozoa has been shown to be inhibited by fluoride, iodoacetate, 2,4-dinitrophenol, and cetyltrimethylammonium bromide. 5. Uptake of K(+) is inhibited by ouabain and half maximum inhibition is obtained with a concentration of 50mmum. There is a slight stimulation of K(+) uptake in the presence of ouabain at about 0.3% of the concentration required for half maximum inhibition.

当犬精液在5摄氏度下储存24小时时，精子会丢失钾离子（K⁺），钠离子（Na⁺）则在细胞内蓄积。2. 如果在冷藏期结束后，精液在添加葡萄糖的情况下于37摄氏度孵育，精子会迅速摄取钾离子并排出钠离子，细胞内钾离子在30分钟内达到最大值。3. 冷藏后的精液在20摄氏度下孵育时，精子在3小时内摄取钾离子，但细胞内钠离子浓度没有变化。4. 已证明氟化物、碘乙酸盐、2,4-二硝基苯酚和十六烷基三甲基溴化铵会抑制犬精子排出钠离子和摄取钾离子。5. 哇巴因会抑制钾离子摄取，浓度为50μM时可获得半数最大抑制效果。在约为半数最大抑制所需浓度0.3%的哇巴因存在下，钾离子摄取会有轻微刺激。

Quinn P J, White I G

Biochem J. 1967 Aug;104(2):328-31. doi: 10.1042/bj1040328.

When dog semen is stored at 5 degrees for 24hr., K(+) is lost from the spermatozoa and Na(+) accumulates in the cells. 2. If at the end of the cold-storage period the semen is incubated at 37 degrees in the presence of added glucose there is a rapid uptake of K(+) and extrusion of Na(+) from the spermatozoa, the intracellular K(+) reaching a maximum within 30min. 3. When the semen is incubated at 20 degrees after cold storage there is an uptake of K(+) by the spermatozoa over 3hr. but no change in intracellular Na(+) concentration. 4. The extrusion of Na(+) and uptake of K(+) by dog spermatozoa has been shown to be inhibited by fluoride, iodoacetate, 2,4-dinitrophenol, and cetyltrimethylammonium bromide. 5. Uptake of K(+) is inhibited by ouabain and half maximum inhibition is obtained with a concentration of 50mmum. There is a slight stimulation of K(+) uptake in the presence of ouabain at about 0.3% of the concentration required for half maximum inhibition.

当犬精液在5摄氏度下储存24小时时，精子会丢失钾离子（K⁺），钠离子（Na⁺）则在细胞内蓄积。2. 如果在冷藏期结束后，精液在添加葡萄糖的情况下于37摄氏度孵育，精子会迅速摄取钾离子并排出钠离子，细胞内钾离子在30分钟内达到最大值。3. 冷藏后的精液在20摄氏度下孵育时，精子在3小时内摄取钾离子，但细胞内钠离子浓度没有变化。4. 已证明氟化物、碘乙酸盐、2,4-二硝基苯酚和十六烷基三甲基溴化铵会抑制犬精子排出钠离子和摄取钾离子。5. 哇巴因会抑制钾离子摄取，浓度为50μM时可获得半数最大抑制效果。在约为半数最大抑制所需浓度0.3%的哇巴因存在下，钾离子摄取会有轻微刺激。