钙刺激下乙醇胺和丝氨酸掺入家蝇（Musca domestica）磷脂的过程。
The calcium-stimulated incorporation of ethanolamine and serine into the phospholipids of the housefly Musca domestica.
作者信息
Crone H D
出版信息
Biochem J. 1967 Aug;104(2):695-704. doi: 10.1042/bj1040695.
Abstract
- The calcium-stimulated incorporation of [2-(14)C]ethanolamine and l-[3-(14)C]-serine into the phospholipids of homogenates of the fat bodies from larval houseflies (Musca domestica) was studied. 2. Ethanolamine and serine acted as competitive inhibitors with one another. N-Methylethanolamine was not distinguished from ethanolamine by the system. Tris buffer was also a competitor with these compounds, and a number of other amino alcohols were inhibitory, probably competitively. 3. The incorporation of [(32)P]phosphorylethanolamine into phospholipids was observed in suspensions of whole fat bodies. This incorporation was stimulated by magnesium. 4. During the incubation of the homogenates, a calcium-stimulated breakdown of phospholipids by a phospholipase A occurred. 5. These results are compared with results published for similar mammalian systems, and their possible physiological significance is discussed.
摘要
- 研究了钙刺激下[2-(14)C]乙醇胺和L-[3-(14)C]丝氨酸掺入家蝇幼虫脂肪体匀浆磷脂中的情况。2. 乙醇胺和丝氨酸彼此作为竞争性抑制剂起作用。该系统无法区分N-甲基乙醇胺和乙醇胺。Tris缓冲液也是这些化合物的竞争者，其他一些氨基醇也有抑制作用，可能是竞争性的。3. 在整个脂肪体的悬浮液中观察到[(32)P]磷酸乙醇胺掺入磷脂中。这种掺入受到镁的刺激。4. 在匀浆孵育过程中，磷脂酶A引起了钙刺激的磷脂分解。5. 将这些结果与针对类似哺乳动物系统发表的结果进行了比较，并讨论了它们可能的生理意义。
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