钙刺激下乙醇胺和丝氨酸掺入家蝇（Musca domestica）磷脂的过程。
The calcium-stimulated incorporation of ethanolamine and serine into the phospholipids of the housefly Musca domestica.
作者信息
Crone H D
出版信息
Biochem J. 1967 Aug;104(2):695-704. doi: 10.1042/bj1040695.
Abstract
- The calcium-stimulated incorporation of [2-(14)C]ethanolamine and l-[3-(14)C]-serine into the phospholipids of homogenates of the fat bodies from larval houseflies (Musca domestica) was studied. 2. Ethanolamine and serine acted as competitive inhibitors with one another. N-Methylethanolamine was not distinguished from ethanolamine by the system. Tris buffer was also a competitor with these compounds, and a number of other amino alcohols were inhibitory, probably competitively. 3. The incorporation of [(32)P]phosphorylethanolamine into phospholipids was observed in suspensions of whole fat bodies. This incorporation was stimulated by magnesium. 4. During the incubation of the homogenates, a calcium-stimulated breakdown of phospholipids by a phospholipase A occurred. 5. These results are compared with results published for similar mammalian systems, and their possible physiological significance is discussed.
摘要
- 研究了钙刺激下[2-(14)C]乙醇胺和L-[3-(14)C]丝氨酸掺入家蝇幼虫脂肪体匀浆磷脂中的情况。2. 乙醇胺和丝氨酸彼此作为竞争性抑制剂起作用。该系统无法区分N-甲基乙醇胺和乙醇胺。Tris缓冲液也是这些化合物的竞争者，其他一些氨基醇也有抑制作用，可能是竞争性的。3. 在整个脂肪体的悬浮液中观察到[(32)P]磷酸乙醇胺掺入磷脂中。这种掺入受到镁的刺激。4. 在匀浆孵育过程中，磷脂酶A引起了钙刺激的磷脂分解。5. 将这些结果与针对类似哺乳动物系统发表的结果进行了比较，并讨论了它们可能的生理意义。
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本文引用的文献
1
Metabolism of phospholipids. VI. The effect of metal ions on the incorporation of L-serine into phosphatidylserine.
Biochim Biophys Acta. 1962 Mar 12;57:555-61. doi: 10.1016/0006-3002(62)91163-0.
2
THE REPLACEMENT OF LIPID-BOUND CHOLINE BY OTHER BASES IN THE PHOSPHOLIPIDS OF THE HOUSEFLY, MUSCA DOMESTICA.
J Insect Physiol. 1965 Mar;11:225-36. doi: 10.1016/0022-1910(65)90070-3.
3
THE PHOSPHOLIPIDS OF THE HOUSEFLY, MUSCA DOMESTICA.
Biochem J. 1963 Oct;89(1):11-21. doi: 10.1042/bj0890011.
4
A hydrolytic procedure for the identification and estimation of individual phospholipids in biological samples.
Biochem J. 1960 Apr;75(1):45-53. doi: 10.1042/bj0750045.
5
The use of amine buffers in studies with enzymes.
Ann N Y Acad Sci. 1961 Jun 17;92:426-39. doi: 10.1111/j.1749-6632.1961.tb44992.x.
6
Metabolism of phospholipids. III. The effect of calcium ions on the incorporation of labelled choline into rat-liver microsomes.
Biochim Biophys Acta. 1961 Jan 29;46:505-13. doi: 10.1016/0006-3002(61)90581-9.
7
The function of cytidine coenzymes in the biosynthesis of phospholipides.
J Biol Chem. 1956 Sep;222(1):193-214.
8
The relationship between phosphatide serine and ethanolamine in larvae of the housefly, Musca domestica.
J Insect Physiol. 1967 Jan;13(1):81-90. doi: 10.1016/0022-1910(67)90005-4.
9
The larval fat body of Sarcophaga bullata (Diptera) as a system for studying phospholipid biosynthesis.
J Insect Physiol. 1966 Jun;12(6):619-24. doi: 10.1016/0022-1910(66)90107-7.
10
Metabolism of aldrin and dihydroaldrin by houseflies (M. Domestica L.) in vivo and by housefly and pig liver microsomes.
Life Sci. 1966 Dec;5(24):2315-20. doi: 10.1016/0024-3205(66)90068-3.
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