Strandberg K, Sydbom A, Uvnäs B
Acta Physiol Scand. 1975 May;94(1):54-62. doi: 10.1111/j.1748-1716.1975.tb05861.x.
The incorporation of labelled phospholipid precursors, [Me-14C]-choline, L-[3(-14)C]-serine, [2(-14)C]-ethanolamine and [2(-3)H]-myoinositol into the phospholipids of isolated rat mast cells was studied. The label from the different precursors were found to be essentially associated with compounds with the t.1.c.-motility of the respective phospholipids. Whereas the incorporation of [Me(-14)c]-choline and L-[3(-14)C]-serine showed evidence of saturation the incorporation of [2(-14)C]-ethanolamine was linear with time (2 h) and it was not saturated by increasing the concentration from 0.07 mM to 2.07 mM. The incorporation of [2(-3)H]-myoinositol was stimulated by Ca2+ (1 mM) or Mg2+ (1 mM), while the incorporation of the other precursors was stimulated only in the presence of both Ca2+ and Mg2+ (1 mM). Antimycin A (1muM), an inhibitor of the respiratory chain, significantly ingibited the incorporation of [Me(-14)c]-choline, L-[3(-14)C]-serine and [2(-3)H]-myoinositol but not that of [2(-14)C]-ethanolamine. The experimental system used might be a useful model for studies on the turnover of membrane phospholipids during histamine release.
研究了标记的磷脂前体[甲基 - 14C] - 胆碱、L - [3(-14)C] - 丝氨酸、[2(-14)C] - 乙醇胺和[2(-3)H] - 肌醇掺入分离的大鼠肥大细胞磷脂中的情况。发现来自不同前体的标记物基本上与具有相应磷脂薄层层析迁移率的化合物相关。虽然[甲基(-14)C] - 胆碱和L - [3(-14)C] - 丝氨酸的掺入显示出饱和迹象，但[2(-14)C] - 乙醇胺的掺入随时间（2小时）呈线性，并且将浓度从0.07 mM增加到2.07 mM时未饱和。[2(-3)H] - 肌醇的掺入受到Ca2 +（1 mM）或Mg2 +（1 mM）的刺激，而其他前体的掺入仅在同时存在Ca2 +和Mg2 +（1 mM）时受到刺激。呼吸链抑制剂抗霉素A（1μM）显著抑制[甲基(-14)C] - 胆碱、L - [3(-14)C] - 丝氨酸和[2(-3)H] - 肌醇的掺入，但不抑制[2(-14)C] - 乙醇胺的掺入。所使用的实验系统可能是研究组胺释放过程中膜磷脂周转的有用模型。
