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极高磁场中的酶-底物反应。II.

Enzyme-substrate reactions in very high magnetic fields. II.

作者信息

Rabinovitch B, Maling J E, Weissbluth M

出版信息

Biophys J. 1967 Jul;7(4):319-27. doi: 10.1016/S0006-3495(67)86591-3.

DOI:10.1016/S0006-3495(67)86591-3
PMID:6048871
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1368063/
Abstract

We have examined the effect of conditioning the enzyme trypsin in solution at pH 8.2 in a large magnetic field before determining its reactivity towards a synthetic substrate N-benzoyl DL-arginine p-nitroanilide (BAPA). This "pretreatment" was allowed to proceed for as long as 3(2/3) hr in a magnetic field of 208 kgauss at temperatures 26 and 36 degrees C. No effect on reactivity was observed when such pretreated enzyme solutions were compared with identical but untreated enzyme solutions. A single such reaction, allowed to proceed directly in a magnetic field of 220 kgauss for 9 min, similarly showed no difference in rate from its control.

摘要

在测定胰蛋白酶对合成底物N-苯甲酰-DL-精氨酸对硝基苯胺（BAPA）的反应活性之前，我们研究了在pH 8.2的溶液中，于强磁场条件下对胰蛋白酶进行预处理的效果。这种“预处理”在26℃和36℃的温度下，于208千高斯的磁场中持续进行长达3(2/3)小时。将经过这种预处理的酶溶液与相同但未处理的酶溶液相比较时，未观察到对反应活性有任何影响。让一个这样的反应直接在220千高斯的磁场中进行9分钟，同样显示其反应速率与对照无差异。

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引用本文的文献

1
Investigation of magnetic field effect on trypsin activity.磁场对胰蛋白酶活性影响的研究。
Radiat Environ Biophys. 1980;18(4):275-80. doi: 10.1007/BF01324270.

本文引用的文献

1
EFFECT OF A MAGNETIC FIELD ON CARBOXYDISMUTASE.
Nature. 1964 May 2;202:452-4. doi: 10.1038/202452a0.
2
The preparation and properties of two new chromogenic substrates of trypsin.两种新型胰蛋白酶显色底物的制备与性质
Arch Biochem Biophys. 1961 Nov;95:271-8. doi: 10.1016/0003-9861(61)90145-x.
3
Enzyme-substrate reactions in very high magnetic fields. I.极高磁场中的酶-底物反应。I.
Biophys J. 1967 Mar;7(2):187-204. doi: 10.1016/S0006-3495(67)86583-4.
4
Enzyme substrate reactions in high magnetic fields.高磁场中的酶底物反应。
Biophys J. 1965 Nov;5(6):767-76. doi: 10.1016/S0006-3495(65)86751-0.

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