Papageorgiou G
Biophys J. 1967 Jul;7(4):375-89. doi: 10.1016/S0006-3495(67)86595-0.
The intensity of the "steady-state" fluorescence of "aerobic" Anacystis nidulans is variable under prolonged illumination with orange (590 mmu) or blue (440 mmu) light for both normally photosynthesizing and DCMU-poisoned cells. In general, orange light illumination causes an increase of the fluorescence intensity followed by a decrease, while blue light causes an increase until a steady level is reached. Poisoned Anacystis cells show four to eight times larger changes in fluorescence intensity than the normal cells; the detailed time course of fluorescence changes is also different in poisoned and normal cells. When algae are cooled to -196 degrees C in light, the light-induced changes in the "steady-state" fluorescence disappear in both types of cells. Difference fluorescence spectra, constructed by subtracting the fluorescence spectra taken after 5-15 min of illumination from those after 60-90 min of illumination, show a doublet structure of the difference band with a major peak coinciding with the Anacystis emission maximum (685 mmu) and a minor peak located at about 693 mmu.
在橙色（590 毫微米）或蓝色（440 毫微米）光的长时间照射下，对于正常进行光合作用的细胞和被二氯苯基二甲基脲（DCMU）毒害的细胞，“需氧”集胞藻的“稳态”荧光强度是可变的。一般来说，橙色光照射会使荧光强度先增加后降低，而蓝光会使荧光强度增加直至达到稳定水平。被毒害的集胞藻细胞的荧光强度变化比正常细胞大 4 至 8 倍；在被毒害的细胞和正常细胞中，荧光变化的详细时间进程也有所不同。当藻类在光照下冷却至 -196℃时，两种类型细胞中光诱导的“稳态”荧光变化都会消失。通过从照射 60 - 90 分钟后的荧光光谱中减去照射 5 - 15 分钟后的荧光光谱构建的差异荧光光谱显示，差异带具有双峰结构，一个主峰与集胞藻的发射最大值（685 毫微米）重合，一个小峰位于约 693 毫微米处。
