禽类肝脏组织在体外对16-表雌三醇和雌三醇的相互转化
Interconversion of 16-epioestriol and oestriol by avian liver tissue in vitro.
作者信息
Raud H R, Hobkirk R
出版信息
Biochem J. 1967 Jun;103(3):724-9. doi: 10.1042/bj1030724.
Abstract
- [16-(14)C]16-Epioestriol and [6,7-(3)H(2)]oestriol were incubated both simultaneously and separately at 40 degrees with hen liver homogenates obtained from birds in the laying stage. 2. Purification and identification of products was made by ether extraction, Girard separation, Celite partition and paper chromatography and crystallization to constant specific activity both with and without derivative formation. Percentage conversions of the radioactive 16-epioestriol and oestriol were calculated from these specific activities. 3. Oestriol yielded 16-epioestriol as early as 5min. after exposure of steroid to the tissue. No production of oestriol from 16-epioestriol was detectable in this time. 4. Considerably more 16-epioestriol was formed from oestriol than oestriol by the reverse reaction after 90min. of incubation. 5. 16-Oxo-oestradiol-17beta appeared in all cases to be the major identified intermediate in the interconversion of 16-epioestriol and oestriol.
摘要
将[16-(14)C]16-表雌三醇和[6,7-(3)H(2)]雌三醇分别与产蛋期母鸡肝脏匀浆在40℃同时孵育。
通过乙醚萃取、吉拉德分离、硅藻土分配、纸色谱法以及结晶法对产物进行纯化和鉴定，无论是否形成衍生物，均使其比活度恒定。根据这些比活度计算放射性16-表雌三醇和雌三醇的转化率。
雌三醇在甾体与组织接触后5分钟就产生了16-表雌三醇。在此期间未检测到16-表雌三醇生成雌三醇。
孵育90分钟后，由雌三醇生成的16-表雌三醇明显多于由16-表雌三醇通过逆反应生成的雌三醇。
在所有情况下，16-氧代-雌二醇-17β似乎都是16-表雌三醇和雌三醇相互转化过程中主要的已鉴定中间体。
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