人妊娠晚期尿液的雌激素缀合物。

Oestrogen conjugates of human late-pregnancy urine.

Smith E R, Kellie A E

Biochem J. 1967 Jul;104(1):83-94. doi: 10.1042/bj1040083.

Abstract

The separation of the oestrogen conjugates in late-pregnancy urine into two groups, peaks I and II, by gel filtration on Sephadex G-25 (Beling, 1963) has been shown to be affected by the presence of urate, which delays the elution of peak II conjugates. 2. By reapplication to a Sephadex column, peak I conjugates have been further separated into two groups (peaks IA and IB) and the metabolites in urine effecting this separation have been studied. 3. Further analysis of the mixed conjugates in the main groups IA, IB and II by ion-exchange and partition chromatography has led to the identification of some of the conjugates present. 4. Oestriol 3-sulphate 16alpha-glucuronide and 16alpha-hydroxyoestrone 3-sulphate 16alpha-glucuronide have been identified in peak IA. 5. The main components of peak IB have been identified as oestrone 3-glucuronide and oestriol 3-glucuronide. 6. The major conjugate in peak II was oestriol 16alpha-glucuronide and no oestriol 17beta-glucuronide was found; small amounts of the ring-d monoglucuronides oestradiol 17beta-glucuronide, 16-epioestriol 16beta-glucuronide and 16alpha-hydroxyoestrone 16alpha-glucuronide were found in this fraction. 7. The behaviour of synthetic oestrogen monoglucuronides has been used as a guide in separation.

摘要

通过在葡聚糖凝胶G - 25上进行凝胶过滤，将妊娠晚期尿液中的雌激素结合物分为两组，即峰I和峰II（贝林，1963年），结果表明尿酸盐的存在会影响这种分离，它会延迟峰II结合物的洗脱。2. 通过重新应用于葡聚糖凝胶柱，峰I结合物进一步分为两组（峰IA和峰IB），并对尿液中导致这种分离的代谢物进行了研究。3. 通过离子交换和分配色谱对主要组IA、IB和II中的混合结合物进行进一步分析，已鉴定出其中存在的一些结合物。4. 在峰IA中已鉴定出雌三醇3 - 硫酸盐16α - 葡萄糖醛酸苷和16α - 羟基雌酮3 - 硫酸盐16α - 葡萄糖醛酸苷。5. 峰IB的主要成分已鉴定为雌酮3 - 葡萄糖醛酸苷和雌三醇3 - 葡萄糖醛酸苷。6. 峰II中的主要结合物是雌三醇16α - 葡萄糖醛酸苷，未发现雌三醇17β - 葡萄糖醛酸苷；在该部分中发现了少量的环D单葡萄糖醛酸苷，即雌二醇17β - 葡萄糖醛酸苷、16 - 表雌三醇16β - 葡萄糖醛酸苷和16α - 羟基雌酮16α - 葡萄糖醛酸苷。7. 合成雌激素单葡萄糖醛酸苷的行为已被用作分离的指导。