Howarth B, Torregrossa D, Britton W M
Poult Sci. 1977 Jul;56(4):1265-8. doi: 10.3382/ps.0561265.
Chicken and turkey spermatozoal lipids were separated into acidic and non-acidic fractions by DEAE cellulose column chromatography. The phospholipid composition of each fraction was examined by thin-layer chromatography, followed by phosphorus analysis. Chicken sperm phospholipid comprised 38.21% phosphatidyl choline, 9.04% phosphatidal choline, 12.62% phosphatidyl ethanolamine, 8.04% phosphatidal ethanolamine, 8.28% sphingomyelin, 9.76% phosphatidyl serine, 6.5% phosphatidyl inositol, and 7.49% cardiolipin. Turkey sperm phospholipid comprised 34.15% phosphatidyl choline, 7.14% phosphatidal choline, 15.61% phosphatidyl ethanolamine, 11.34% phosphatidal ethanolamine, 9.91% sphingomyelin, 8.83% phosphatidyl serine, 4.80% phosphatidyl inositol, and 8.22% cardiolipin.
通过二乙氨基乙基纤维素柱色谱法将鸡和火鸡的精子脂质分离为酸性和非酸性组分。通过薄层色谱法检查每个组分的磷脂组成,随后进行磷分析。鸡精子磷脂包含38.21%的磷脂酰胆碱、9.04%的磷脂酰胆碱、12.62%的磷脂酰乙醇胺、8.04%的磷脂酰乙醇胺、8.28%的鞘磷脂、9.76%的磷脂酰丝氨酸、6.5%的磷脂酰肌醇和7.49%的心磷脂。火鸡精子磷脂包含34.15%的磷脂酰胆碱、7.14%的磷脂酰胆碱、15.61%的磷脂酰乙醇胺、11.34%的磷脂酰乙醇胺、9.91%的鞘磷脂、8.83%的磷脂酰丝氨酸、4.80%的磷脂酰肌醇和8.22%的心磷脂。