艾氏腹水癌细胞中载体介导的单糖转运的证据。
Evidence for carrier-mediated transport of monosaccharides in the Ehrlich ascites tumor cell.
作者信息
Kolber A R, LeFevre P G
出版信息
J Gen Physiol. 1967 Aug;50(7):1907-28. doi: 10.1085/jgp.50.7.1907.
Evidence for carrier-mediated transport of monosaccharides in the Ehrlich ascites tumor cells was provided through kinetic analysis of data obtained by: (a) studying sugar uptake by dilute cell suspensions with an optical densimetric apparatus, (b) studying sugar uptake by thicker cell suspensions by means of direct chemical analytical methods using packed cell plugs, (c) observing the effects of a competitive inhibitor upon sugar uptake with the chemical analytical method, and (d) measurement of tracer uptake of a high affinity sugar in thick cell suspensions in the absence of net movement. Quantitative application of the data obtained with the above experimental procedures to theoretical model systems derived for both carrier-mediated transport and simple passive diffusion indicated that the results were consonant with predictions for the carrier-mediated transport model, but could not be explained on the basis of uncomplicated diffusion.
通过对以下方式获得的数据进行动力学分析，提供了埃利希腹水肿瘤细胞中载体介导的单糖转运的证据：(a) 使用光密度测定仪研究稀释细胞悬液对糖的摄取；(b) 通过使用填充细胞塞的直接化学分析方法研究较浓细胞悬液对糖的摄取；(c) 用化学分析方法观察竞争性抑制剂对糖摄取的影响；(d) 在没有净移动的情况下测量浓细胞悬液中高亲和力糖的示踪剂摄取。将上述实验程序获得的数据定量应用于为载体介导的转运和简单被动扩散推导的理论模型系统，结果表明这些结果与载体介导的转运模型的预测一致，但不能用简单扩散来解释。
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