逆流诱导的上坡运输。
Uphill transport induced by counterflow.
作者信息
ROSENBERG T, WILBRANDT W
出版信息
J Gen Physiol. 1957 Nov 20;41(2):289-96. doi: 10.1085/jgp.41.2.289.
Abstract
- In a membrane transport system containing a mobile carrier with affinities for two substrates a concentration gradient with respect to one of the substrates under certain conditions is able to induce an "uphill" transport (against the concentration gradient) of the other. 2. In a kinetic treatment quantitative conditions for such a "flow-induced uphill transport" and some of its characteristics are derived. 3. Experimentally the uphill transport of labelled glucose induced by a concentration gradient for mannose or unlabelled glucose is demonstrated in the human red cell. 4. It is shown that the flow-induced uphill transport is a feature characteristic for mobile carrier systems only and is not to be expected in systems in which the substrate is bound to a fixed membrane component ("adsorption membrane"), although such a system may yield identical transport kinetics. Also with respect to Ussing's flux ratio the two systems are different, the adsorption membrane meeting Ussing's criterion, the carrier membrane not. 5. It is concluded that the transport system in the human red cells must contain a mobile carrier, identical for glucose and mannose.
摘要
- 在一个包含对两种底物具有亲和力的移动载体的膜运输系统中，在某些条件下，相对于其中一种底物的浓度梯度能够诱导另一种底物的“上坡”运输（逆浓度梯度）。2. 在动力学处理中，推导了这种“流动诱导的上坡运输”的定量条件及其一些特性。3. 通过实验证明，在人红细胞中，由甘露糖或未标记葡萄糖的浓度梯度诱导的标记葡萄糖的上坡运输。4. 结果表明，流动诱导的上坡运输是仅移动载体系统特有的特征，在底物与固定膜成分结合的系统（“吸附膜”）中不会出现，尽管这样的系统可能产生相同的运输动力学。同样关于乌辛通量比，这两种系统是不同的，吸附膜符合乌辛标准，载体膜则不符合。5. 得出的结论是，人红细胞中的运输系统必须包含一个对葡萄糖和甘露糖相同的移动载体。
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