小鼠L细胞中的胞质杂种形成：胞质体与细胞比例的影响。

Cybrid formation in mouse L cells: the influence of cytoplast-to-cell ratio.

作者信息

Bunn C L, Eisenstadt J M

出版信息

Somatic Cell Genet. 1977 May;3(3):335-41. doi: 10.1007/BF01538751.

Abstract

The frequency of cybrid colony formation was measured in fusions between enucleated chloramphenicol (CAP)-resistant mouse cells and CAP-sensitive mouse cells in varying ratios. By labeling the CAP-resistant cytoplasts with polystyrene beads and then performing the same fusions with CAP-sensitive cells, the frequency of cybrid fusions could be measured. Comparison of the frequency of viable cybrids (cybrid colonies) with the frequency of cybrid fusions showed that, with increasing fusion ratios of cytoplasts to cells, the proportion of cells fused to cytoplasts increased. Further, the viability of cybrid fusions increased from about 1 in 500 to nearly 1 in 60 over the range of cytoplast-to-cell ratios studied.

摘要

在去核的氯霉素（CAP）抗性小鼠细胞与CAP敏感小鼠细胞以不同比例融合的过程中，测定了杂种细胞集落形成的频率。通过用聚苯乙烯珠子标记CAP抗性细胞质体，然后与CAP敏感细胞进行相同的融合，可以测定杂种细胞融合的频率。将存活杂种细胞（杂种细胞集落）的频率与杂种细胞融合的频率进行比较，结果表明，随着细胞质体与细胞融合比例的增加，与细胞质体融合的细胞比例增加。此外，在所研究的细胞质体与细胞比例范围内，杂种细胞融合的存活率从约500分之一增加到近60分之一。