种间重组细胞和胞质杂种中致瘤性的抑制

Suppression of tumorigenicity in interspecific reconstituted cells and cybrids.

作者信息

Koura M, Isaka H, Yoshida M C, Tosu M, Sekiguchi T

出版信息

Gan. 1982 Aug;73(4):574-80.

Abstract

The effect of cytoplasms on the expression of tumorigenicity was examined in interspecific reconstituted cells and cytoplasmic hybrids (cybrids) formed by fusions between karyoplasts (or intact cells) from highly tumorigenic mouse melanoma B16 cells and cytoplasts from non-tumorigenic, hypoxanthine-guanine phosphoribosyl-transferase (HGPRT)-deficient and chloramphenicol (CAP)-resistant rat myoblastic cells, L6TG X CAPr. The reconstituted clones and cybrid clones isolated in the double selection medium, HAT and CAP, acquired CAP-resistance, and each showed unique morphology and cellular arrangement. Tumorigenicity was suppressed in all the reconstituted clones and cybrid clones examined at an early stage after their isolation, but re-appeared in some clones after prolonged cultivation of the cells.

摘要

通过高致瘤性小鼠黑色素瘤B16细胞的核体（或完整细胞）与非致瘤性、次黄嘌呤 - 鸟嘌呤磷酸核糖转移酶（HGPRT）缺陷且对氯霉素（CAP）耐药的大鼠成肌细胞L6TG X CAPr的细胞质体融合形成的种间重构细胞和细胞质杂种（cybrids），研究了细胞质对致瘤性表达的影响。在双重选择培养基HAT和CAP中分离得到的重构克隆和cybrid克隆获得了对CAP的抗性，并且每个克隆都表现出独特的形态和细胞排列。在所有分离后的早期检测的重构克隆和cybrid克隆中，致瘤性均受到抑制，但在细胞长期培养后，一些克隆中致瘤性又重新出现。