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补体与细胞复合物解离后第三和第四补体成分的物理化学特性

Physico-chemical characteristics of the third and fourth component of complement after dissociation from complement-cell complexes.

作者信息

Dalmasso A P, Müller-Eberhard H J

出版信息

Immunology. 1967 Sep;13(3):293-305.

PMID:6054730
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1409350/
Abstract

The radioactivity labelled third (C′) and fourth (C′) components of human complement were dissociated from erythrocyte membrane—complement complexes. The differential effectiveness of various reagents in causing dissociation suggests that C′ and C′ are bound to membrane sites by hydrophobic bonds. Density gradient ultracentrifugation of the dissociated components showed that they are bound in macromolecular form. Their electrophoretic mobility was greater than that of their native counterparts. In physical and immunochemical properties, dissociated C′ and C′ closely resembled the respective haemolytically inactive conversion products that, during immune haemolysis, accumulate in the fluid phase. It is concluded that in immune haemolysis C′ and C′ become linked to membrane sites only following modification by their respective converting enzymes.

摘要

人类补体的放射性标记第三成分（C′）和第四成分（C′）从红细胞膜 - 补体复合物中解离出来。各种试剂在引起解离方面的不同效果表明，C′和C′通过疏水键与膜位点结合。对解离成分进行密度梯度超速离心表明它们以大分子形式结合。它们的电泳迁移率大于其天然对应物。在物理和免疫化学性质方面，解离的C′和C′与各自在免疫溶血过程中积聚在液相中的溶血无活性转化产物非常相似。得出的结论是，在免疫溶血中，C′和C′仅在被各自的转化酶修饰后才与膜位点相连。

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