Kappy M S, Metzenberg R L
J Bacteriol. 1967 Nov;94(5):1629-37. doi: 10.1128/jb.94.5.1629-1637.1967.
A temperature-conditional lethal mutant of Neurospora crassa, un-t (55701), was resistant to neutral amino acid analogues by virtue of a decreased ability to transport these analogues and their natural congeners across the cell membrane. The uptake of acidic, but not basic, amino acids was also impaired, as was the uptake of potassium ions. After preincubation above the tolerated temperature, the ability to take up a still wider variety of metabolites was greatly reduced. Protoplasts of the mutant were more osmotically fragile than those of wild type. The possibility that the mutant has a generalized membrane defect is discussed.
粗糙脉孢菌的一个温度条件致死突变体un-t(55701)，由于其跨细胞膜转运这些类似物及其天然同源物的能力降低，对中性氨基酸类似物具有抗性。酸性氨基酸（而非碱性氨基酸）的摄取以及钾离子的摄取也受到损害。在耐受温度以上进行预孵育后，摄取更多种类代谢物的能力大大降低。该突变体的原生质体比野生型原生质体在渗透压方面更脆弱。文中讨论了该突变体存在普遍膜缺陷的可能性。
