Lee J C, Ingram V M
Science. 1967 Dec 8;158(3806):1330-2. doi: 10.1126/science.158.3806.1330.
Radioactive aminoacyl transfer RNA's isolated from erythrocytes in the blood of 4-day-old chick embryos and from reticulocytes of adult chickens were analyzed by chromatography on methylated albumin kieselguhr and freon columns. Embryonic and adult methionyl transfer RNA's showed qualitative and quantitative differences in both chromatographic systems. The patterns for arginyl, seryl, and tyrosyl transfer RNA's in the two cell types were similar, while the leucyl transfer RNA patterns suggested a difference.
从4日龄鸡胚血液中的红细胞以及成年鸡的网织红细胞中分离出的放射性氨酰转移RNA,通过在甲基化白蛋白硅藻土柱和氟利昂柱上进行色谱分析。胚胎期和成年期的甲硫氨酰转移RNA在两种色谱系统中均表现出定性和定量的差异。两种细胞类型中精氨酰、丝氨酰和酪氨酰转移RNA的图谱相似,而亮氨酰转移RNA的图谱则显示存在差异。